Anatomy, Histologyو Embryology
elham hoveizi; Kiavash Hushmandi
Volume 29, Issue 3 , September and October 2022, , Pages 330-343
Abstract
Introduction: In recent years, researchers have considered the anticancer activity coumarins, due to their powerful biological activity and poor toxicity that can neutralize the side effects induced by radiotherapy. The aim of this study was to investigate the cytotoxic effects of coumarin on HT-29 and ...
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Introduction: In recent years, researchers have considered the anticancer activity coumarins, due to their powerful biological activity and poor toxicity that can neutralize the side effects induced by radiotherapy. The aim of this study was to investigate the cytotoxic effects of coumarin on HT-29 and A549 cancer cells.
Materials and Methods: In this experimental study, the stoke of coumarin was prepared and then for 1,3, and 5 days at concentrations of5, 10, 15, 20, and 25 μM the cells were treated and evaluated on viability days and morphology of the cells indefinite days. The IC50 concentration of coumarin was calculated using MTT assay in two cell lines. Also, the expression of the involved genes in apoptosis such as Bax, Bad, and Bcl-2 was evaluated by the qRT_PCR method. Data were analyzed by a one-way ANOVA test.
Results: The results showed that coumarin reduced the viability and proliferation of HT-29 and special A549 cells by dose and time significantly (P≤0.001), as well as the viability rate of cells in treated cells on the fifth day, significantly decreased compared to the control group (P <0.05). Morphological changes such as reduced chromatin density, cell turnover were also noticeably observed in the cells. Also, molecular results showed that coumarin could significantly increase the expression of Bax, Bad genes and decrease the expression of Bcl-2 gene expression. That these genetic changes in A549 cells were significantly greater than HT-29.
Conclusion: Coumarin is capable of anti-proliferative activity and induces apoptosis effectively against colon and lung cancer cells.
Biotechnology & nanotechnology
Akbar Safipour Afshar; fateme Saied Nematpour
Volume 27, Issue 4 , November and December 2020, , Pages 550-558
Abstract
Introduction: Green synthesis of silver nanoparticles is a simple and low-cost method. In addition, finding new therapeutic effects for silver nanoparticles is considered as an attractive field in cancer research. In the present study, synthesis of silver nanoparticles was performed by aqueous leaf extract ...
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Introduction: Green synthesis of silver nanoparticles is a simple and low-cost method. In addition, finding new therapeutic effects for silver nanoparticles is considered as an attractive field in cancer research. In the present study, synthesis of silver nanoparticles was performed by aqueous leaf extract of barberry. The effects of Ag nanoparticles were evaluated on cell viability and expression levels of ODC1 gene of the human breast cancer cell line (MDA-MB-231).
Materials and Methods: The synthetic nanoparticles were analyzed by UV-vis spectrophotometry and Transmission Electron Microscopy. The cultured cancer cells were treated under different concentrations of synthesized nanoparticles.The viability of the cells was analyzed by MTT assay and relative expression of ODC1 gene by Real-Time PCR.
Results: TEM photomicrographs showed that the spherical silver nanoparticles have been synthesized with an average size of 16 nm. The results showed cytotoxicity of silver nanoparticles in a time and dose-dependent manner. The average IC50 calculated was 16.48 µg/ml. The results also showed that silver nanoparticles decreased ODC1 gene expression levels about 4 folds at 20 µg/ml nanoparticles compared to control.
Conclusion: It seems that green synthesized silver nanoparticles through the gradual release of silver ions in the acidic environment of the cancer cells and production of oxygen radicals, as well as reduction of ODC1gene expression cause diminish of cancer cell viability.
Microbiology
Morteza Yazdani; Fereshteh Jookar Kashi; Akram Rahimi-Moghaddam
Volume 26, Issue 5 , November and December 2019, , Pages 555-565
Abstract
Background: Considering the adverse effects of synthetic antioxidants, anticancer drugs and antibiotics on human health, in this study, bioactivities of Mentha longifolia essential oils collected from Marivan and Qaza An were examined and compared with each other for identification a natural alternative ...
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Background: Considering the adverse effects of synthetic antioxidants, anticancer drugs and antibiotics on human health, in this study, bioactivities of Mentha longifolia essential oils collected from Marivan and Qaza An were examined and compared with each other for identification a natural alternative of synthetic antioxidants, anticancer drugs and antibiotics.Materials and Methods: Essential oil of M. longifolia leaves and stems collected from Marivan and Qaza An was prepared using solvent-free microwave extraction method. The essential oil components were identified by gas chromatography/mass spectrometry. The antioxidant and cytotoxicity activity of the essential oils were determined via β–carotene bleaching assay and brine shrimp lethality test, respectively. The antimicrobial activity of the essential oils was evaluated by the agar well diffusion method and by determination of minimum inhibitory concentration.Results: The main components of M. longifolia essential oil collected from Marivan were pulegone (81.45%) and isopulegol (8.39%) and the main components of M. longifolia essential oil collected from Qaza An were pulegone (48.29%) and piperitenone oxide (23.53%). The inhibition of linoleic acid oxidation by M. longifolia essential oils collected from Marivan, Qaza An and BHT were 80.02%, 78.59% and 95.50%, respectively. The LC50 of the M. longifolia essential oils collected from Marivan, Qaza An and vincristine sulfate were 53.47μg/ml, 55.96 μg/ml and 0.751 μg/ml, respectively. M. longifolia essential oils had good antimicrobial activity, especially against gram-positive bacteria.Conclusion: M. longifolia essential oils have significant antioxidant, antimicrobial and anticancer activity and can be introduced as alternatives of synthetic antioxidants, antibiotics and anti-cancer drugs.
Bagher Seyyed Alipour; Moghaddeseh Fazeli; Salman Ahmadi Asb Chin; Hamid Cheshomi; Leila Saadat Aldaghi
Volume 22, Issue 5 , November and December 2015, , Pages 919-926
Abstract
Background and objective: Chalcones are belonged to an important group of flavonoids which has proven anti-inflammatory and antineoplastic effects. Assessing cellular Cytotoxicity effect of new synthetic chalcone on HeLa cell line is the aim of present study. Materials and Methods: In this experimental ...
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Background and objective: Chalcones are belonged to an important group of flavonoids which has proven anti-inflammatory and antineoplastic effects. Assessing cellular Cytotoxicity effect of new synthetic chalcone on HeLa cell line is the aim of present study. Materials and Methods: In this experimental in vitro study, HeLa cells were treated with various concentrations of chalcone for 72 hours and cell death rates was evaluated by MTT test. Results were analyzed by Student T-Test and morphological changes were assessed by Acridine orange and propidium iodide (PI) staining. Results: Our results showed that new synthetic chalcone in studied concentrations inhibited tumor cell growth. Highest percent (50.44%) of growth inhibition was seen in 40 µg/ml concentration and after 72 hours IC50 was 40µg/ml. morphological changes of HeLa cells after 24 hours were also strengthened the probability of cellular apoptosis. Conclusion: The results showed that new chalcone has inhibitive effects on HeLa tumor cell line and a probable mechanism of apoptosis can causes cellular death.
Safiyeh Ghazzafi; Ahmad Hamta
Volume 21, Issue 1 , March and April 2015, , Pages 122-130
Abstract
Background: Breast Cancer is the most common cancer in the Iranian women. Fortunately, it is a type of preventable and discernible cancer. The hydroethanolic extract of the leaves and flowered twigs of Thymu vulgaris contain various ingrediants in which the Thymol and Carvacrol are the most important ...
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Background: Breast Cancer is the most common cancer in the Iranian women. Fortunately, it is a type of preventable and discernible cancer. The hydroethanolic extract of the leaves and flowered twigs of Thymu vulgaris contain various ingrediants in which the Thymol and Carvacrol are the most important of them. The effects of this anti-cancer in this extract are attributed significantly to these ingrediants . The objective of this research is to study the comparison of the efficiency of hydroethanolic extract and taxol on 4T1 line cells and their effects rate on the induction of apoptosis in cancer cells.
Materials and Methods: In this research, 4T1 line cells were bought from Tehran's Pasteur institute and the hydroethanolic extract is provided by the Soxhlet’s method. Therefore, We obtained Cytotoxicity five different doses of the extract and five different doses of Taxol in 24, 48 and 72 hours and studied the viability of cells by the MTT and Tripan blue methods. Also, in this research the morphologic changes are done by the Hoechst and Propidiumi odied coloring methods.
Results: Extract and taxol-treated cells at different times have indicated distinct differences and dose-related results. Therefore, simultaneous treatment with the extract in comparison with taxol and taxol-treated cell extract can destroy more cells.
Conclusion: The hydroethanolic extract can increase the cytotoxicity Taxol, Thus it maybe be effective in breast cancer remedy and probably by apoptosis Induction may cause cells death.
Leylasaadat Aldaghi; Abbasali DehpoorJoybari; Farkhondeh Nemati; Reza Mirdashti; Rahim Akrami
Volume 20, Issue 5 , March and April 2014, , Pages 603-610
Abstract
Abstract
Background & objectives: Cancer prevention and treatment by medicinal plants for a long was is one of the most challenging areas of research. Among them, some plants species could suppress or kill tumor cells via apoptosis or necrosis. One of these plants is Astragalus. The purpose of this ...
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Abstract
Background & objectives: Cancer prevention and treatment by medicinal plants for a long was is one of the most challenging areas of research. Among them, some plants species could suppress or kill tumor cells via apoptosis or necrosis. One of these plants is Astragalus. The purpose of this study was investigating the anti-cancer effects of this plant.
Material & method: The cancer cell lines obtained from Pasteur Institute of Iran. The cells were cultured in RPMI 1640. Then the cells have effected from the presence of various concentrations (0.156, 0.312, 0.625, 1.25, 2.5, 5, 7.5 and 10 mg/ml) of Astragalus cystosus. After 72 hours the rate of cytotoxicity was determined by using MTT test. These statistical analyses carried out with a significant level of 0.05 and t-test with excel software.
Findings: These findings indicate that the extract of Astragalus cystosus on Hela cancer cell lines had cytotoxicity in all concentrations and the highest inhibition was 7.5 and 10 mg/ml concentrations. The rate of inhibition in higher concentrations such as 10mg/m1 was equal to 28.98% which is the highest one.
Conclusion: Regarding that the extract of Astragalus cystosus had cytotoxicity on hela cancer cells, further studies can be performed on animal model and clinical trial so that Astragalus used as an anticancer drug.