Shahin Ramazi; Majid Motovalli Bashi; Abolfazl Rad; Hamidreza Khezraee; Morteza Hashemzadeh Chaleshtari
Volume 21, Issue 5 , September and October 2014, , Pages 842-848
Abstract
Background: Allergic rhinitis is a chronic inflammatory disease of the upper airways, this inflammatory response in the nasal mucosa includes an immediate IgE-mediated response. IL-18, a member of the IL-1 family, is known for influencing the balance of IgE level. In present study this problem is investigated ...
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Background: Allergic rhinitis is a chronic inflammatory disease of the upper airways, this inflammatory response in the nasal mucosa includes an immediate IgE-mediated response. IL-18, a member of the IL-1 family, is known for influencing the balance of IgE level. In present study this problem is investigated that whether immunoglobulin (Ig) E levels in serum are associated with -607A/C SNPs of IL18 promoter.
Materials and Methods: Genotyping for -607A/C SNPs of IL18 promoter was performed using 133 patients with AR and 62 healthy control volunteers from Chaharmahal va Bakhtiari province then serum levels of total IgE were determined by ELISA method. Statistical analysis were carried out using T test and ANOVA test by SPSS version 19.
Results: level of total IgE in Serum were significantly greater in allergic rhinitis patients than controls (P=0.017). In addition, the total serum IgE level of the individuals with heterozygous genotype of IL18 (-607AC) were significantly higher regarding other investigated polymorphisms (P=0.045).
Conclusion: According to the results of this study, it seems that the IL18 gene polymorphism -607A/C is associated with IgE levels and susceptibility of allergic rhinitis.
Ahmad Majd; Elham Iziy; Hamid Cheshomi
Volume 21, Issue 3 , July and August 2014, , Pages 451-460
Abstract
Background: Flowers aromatic compounds are the major inhaled allergens. The aim of this research was the comparison of petals allergenicity of in two ontogenetic stages, middle-aged and older of Cytisus scoparius; and Identifying dependence of allergenicity with developmental stages of extremely aromatic ...
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Background: Flowers aromatic compounds are the major inhaled allergens. The aim of this research was the comparison of petals allergenicity of in two ontogenetic stages, middle-aged and older of Cytisus scoparius; and Identifying dependence of allergenicity with developmental stages of extremely aromatic petals.
Materials & Methods: In order to evaluate the petals allergenicity, 18 Hartley guinea pigs were randomly selected and divided into three equal groups. 16% petals buffered extracts were prepared and then subcutaneous injection of extracts and also macroscopic examinations was performed. In order to confirm the petals allergenicity, immunoglobulin E (IgE) levels was measured using ELISA method, and the number of eosinophils and finally blood glucose in guinea pig blood samples was evaluated. Data were analyzed in SPSS16 software using independent t-test.
Results: In guinea pigs treated with middle-aged petal extract, IgE, eosinophil count and also blood glucose significantly increased in comparison to control group, whereas in guinea pigs treated with older petal extract, there was no significant difference between the treatment and control groups. Only, the diameter of wheals in skin test significantly increased in both treatment groups compared to control group (p
Ahamad Majid; Seyyed Mehdi Beheshti Nasr; Elham Izi; Mohammad Mohammadzadeh
Volume 20, Issue 2 , May and June 2013, , Pages 176-183
Abstract
Background: Cytisus scoparius is an ornamental and highly aromatic plant. The petals and stamens in aromatic plants are important allergenic agents. The aim of this study is examination study of allergenicity of petal and stamen in middle-aged ontogenical stage of Cytisus scoparius L. in guinea pig. ...
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Background: Cytisus scoparius is an ornamental and highly aromatic plant. The petals and stamens in aromatic plants are important allergenic agents. The aim of this study is examination study of allergenicity of petal and stamen in middle-aged ontogenical stage of Cytisus scoparius L. in guinea pig.
Methology: In this experimental study, 9 guinea pigs from hartly stirps were randomly selected and divided into three equal groups. Plant extracts were prepared with 16% concentration and injected intraperitoneally to guinea pigs.The first group was considered as negative control (injected with phosphate buffered saline). The second and the third groups received petal extract and petal + stamen extract respectively. In addition intraperitoneal injections within 4 weeks, once per week, subcutaneous injection was performed in the fifth week. Finally, a week after the last injection was taken blood sampling directly from the heart of animals, and the number of eosinophils, immunoglobulin E and blood sugar levels were measured in experimental groups and analyzed data.
Results: Skin test (Created wheal diameter) and serological tests (levels of immunoglobulin E, eosinophils, and blood glucose) in both groups treated with Cytisus scoparius L. significantly increased compared to controls. Electrophoretic profiles was observed about 4 protein bands (2 light protein bands and 2 pale protein bands) in the range of 27 to 85 kD in the middle-aged petal and about 6 more specific protein bands in the range of 10 to 75 kD in middle-aged petal whit stamen proteins.
Conclusion: The results showed that petal of ontogenical stage of middle-aged in Cytisus scoparius have allergenicity property and and this effect is exacerbated when they are together with stamens.