Gyneocology
Farinaz Haghayegh Khorasani; Maryam Zangeneh; Firoozeh Veisi; Maryam Hematti
Abstract
Background: Pregnant women and their fetuses are likely to represent a high‐risk population during the coronavirus disease 2019 (COVID‐19) pandemic. The present study was designed aiming to evaluate the maternal and fetal outcomes in pregnant women diagnosed with COVID-19 with D-dimer levels.Materials ...
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Background: Pregnant women and their fetuses are likely to represent a high‐risk population during the coronavirus disease 2019 (COVID‐19) pandemic. The present study was designed aiming to evaluate the maternal and fetal outcomes in pregnant women diagnosed with COVID-19 with D-dimer levels.Materials and Methods: In this descriptive analysis, blood samples were collected from patients with laboratory-confirmed COVID‐19 for evaluating D-dimer levels. The descriptive statistics are used to give more information using SPSS software.Results: The flowing mean scores were obtained: age (30.99±5.66 y), gravidity (1.89±0.92 %), parity (1.38±0.63 %), and gestational age (24.39±10.6 w). Of 106 eligible pregnant women, 28.3% were receptive in the first trimester, 28.3% in the second trimester, and 43.39% in the third trimester. Our study showed RT-PCR test and HRCT chest scan to be positive in 91.5% and 63.2% of cases, receptively, with clinical suspicion and symptoms. The most common maternal and fetal outcomes included cesarean section, abortion, preterm labor, meconium, pulmonary embolism, prelabor rupture of membranes, and hypertension. The mean level of D-dimer in patients was 852.14±915.08 mg/L. Reference ranges for D-dimers level increased in the trimester order. In pregnant COVID-19 patients, about 79.25% of cases were out of the conventional normal threshold of D-dimer values.Conclusion: The majority of complications were observed in the positive D-dimer levels. D-dimer level elevation continues to be a sign of poor prognosis in pregnant patients with COVID-19.
M NIKBAKHT; R GOLMOHAMMADI
Volume 13, Issue 1 , March and April 2006, , Pages 7-13
Abstract
Background and purpose: Colorectal cancer is one of the most important common cancers all over the world. Its prevalence varies with geographical distribution. Its multifactorial cause may include environmental, genetic and dietary origins. P53 is the most important tumor suppressive gene. P53 exon 6 ...
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Background and purpose: Colorectal cancer is one of the most important common cancers all over the world. Its prevalence varies with geographical distribution. Its multifactorial cause may include environmental, genetic and dietary origins. P53 is the most important tumor suppressive gene. P53 exon 6 mutation with protein overexpression is reported in different areas. This study is intended to determine the relationship between P53 exon 6 mutation with protein overxpression and prognosis in colorectal cancer.
Methods and Materials: This study was conducted on 80 cases of Colorectal cancers, admitted to Isfahan hospitals in Isfahan, Iran from 1382 (2003) to 1385(2006). DNA was extracted by phenol chloroform isoamil alcohol at the Genetic Department of the faculty of Medicine. Exon 6 of the P53 gene was amplified using primers in a PCR assay. After gel electrophoresis by SSCP method, exon 6 mutations were determined. P53 protein overexpression was determined in cases by immunohistochemistry. Chi-square test was used for data analysis.
Results: Mutations were observed in 12 cases (15%); in 10 cases, mutations demonstrated protein overexpression as well. No significant relationship was observed between P53 exon 6 mutation and its overexpression (p>0.05).
Conclusion: The study revealed that not all P53 exon 6 mutations were accompanied by P53 overexpression. Therefore, besides immuno histochemistry, we need PCR-SSCP or sequencing for diagnosis.