Biotechnology & nanotechnology
Ali Ghorbani Ranjbary; Jalil Mehrzad; Hesam Dehghani; Abbas Abdollahi; Saman Hosseinkhani
Volume 29, Issue 1 , May and June 2022, , Pages 141-153
Abstract
Background Colorectal cancer (CRC) is one of the most common cancers in the world. The role of IL-17A in cancer begins in the early stages of tumorigenesis and appears to play an important role in tumorigenesis by causing inflammation. The present study was conducted to investigate the expression of ...
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Background Colorectal cancer (CRC) is one of the most common cancers in the world. The role of IL-17A in cancer begins in the early stages of tumorigenesis and appears to play an important role in tumorigenesis by causing inflammation. The present study was conducted to investigate the expression of KI67 and P53 genes in HT-29 colon epithelial cells with IL-17A.Materials and Methods IL-17A was purchased and 50 ng/ml was added to HT-29 culture medium and after 24 hours the cells were isolated from the culture medium and cell necrosis was examined by MTT. Then RNA was extracted and the expression levels of P53 and KI67 were analyzed using newly designed primers by Reverse transcription (RT) qPCR method and GeniX6 software.Results The MTT test showed that a concentration of 150 ng/ml for 24 hours had maximal necrosis rate in HT-29. After 24 hours of IL-17A incubation, the expression of KI67 (P = 0.003) and P53 (P = 0.001) genes in HT-29 cells in the IL-17A exposed group decreased and increased, respectively. Also, compared to the control group the number of examined HT-29 cells in the IL-17A treated group showed a significant decrease (P
M NIKBAKHT; R GOLMOHAMMADI
Volume 13, Issue 1 , March and April 2006, , Pages 7-13
Abstract
Background and purpose: Colorectal cancer is one of the most important common cancers all over the world. Its prevalence varies with geographical distribution. Its multifactorial cause may include environmental, genetic and dietary origins. P53 is the most important tumor suppressive gene. P53 exon 6 ...
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Background and purpose: Colorectal cancer is one of the most important common cancers all over the world. Its prevalence varies with geographical distribution. Its multifactorial cause may include environmental, genetic and dietary origins. P53 is the most important tumor suppressive gene. P53 exon 6 mutation with protein overexpression is reported in different areas. This study is intended to determine the relationship between P53 exon 6 mutation with protein overxpression and prognosis in colorectal cancer.
Methods and Materials: This study was conducted on 80 cases of Colorectal cancers, admitted to Isfahan hospitals in Isfahan, Iran from 1382 (2003) to 1385(2006). DNA was extracted by phenol chloroform isoamil alcohol at the Genetic Department of the faculty of Medicine. Exon 6 of the P53 gene was amplified using primers in a PCR assay. After gel electrophoresis by SSCP method, exon 6 mutations were determined. P53 protein overexpression was determined in cases by immunohistochemistry. Chi-square test was used for data analysis.
Results: Mutations were observed in 12 cases (15%); in 10 cases, mutations demonstrated protein overexpression as well. No significant relationship was observed between P53 exon 6 mutation and its overexpression (p>0.05).
Conclusion: The study revealed that not all P53 exon 6 mutations were accompanied by P53 overexpression. Therefore, besides immuno histochemistry, we need PCR-SSCP or sequencing for diagnosis.