Fatemeh Zihreh; Siam Nasri; Parisa Karishchi
Volume 22, Issue 2 , May and June 2015, , Pages 377-386
Abstract
Background and purpose: Antioxidants such as Quercetin protect sperm cells from free radical damage. This research was conducted to investigate the effects of Quercetin on spermatogenesis in male rats.
Materials and Methods: In this experimental study 50 male rats were used. Quercetin was injected daily ...
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Background and purpose: Antioxidants such as Quercetin protect sperm cells from free radical damage. This research was conducted to investigate the effects of Quercetin on spermatogenesis in male rats.
Materials and Methods: In this experimental study 50 male rats were used. Quercetin was injected daily at doses of 5, 10, 15, 20 (mg/kg) for two weeks intraperitoneally. One week after the last injection, blood samples were collected and the left testis of rats were removed and weighed. Epididymal sperm and testicular sections were also studied. Data were analyzed by using one-way ANOVA at the significant level of 0.05 in SPSS 19 software.
Results: No significant difference was found in serum level of gonadotropins, but testosterone has increased at dose of 20 mg/kg (6.52±0.50, P=0/0079).Significant difference in testicular weight wasn’t seen. The number of sperm (51.35±1.68, P=0.0251) and also sperm mobility (73.88±5.91, P= 0.0002) in the epididymis especially at dose of 20 mg/kg has increased. No significant difference was found between the groups in the number of spermatogonia cells. But the number of primary spermatocytes (118.25±7.04, P=0.0171), spermatids (106.75±6.28, P=0.0032) and Spermatozoa (101.75±6.30, P= 0.0008) were increased at dose of 20 mg/kg.
Conclusion: No significant change in gonadotropins was seen, which indicates that probably Quercetin has not affected PG axis. Quercetin is believed to improve the quality and quantity of germ cells with its antioxidant effects and increasing of testosterone.
Hadi Tavakkoli Kazeroni; Seyyed Ebrahim Hosseini; Mehrdad Shariati
Volume 21, Issue 3 , July and August 2014, , Pages 514-521
Abstract
Background: Hops (Humulus lupulus L.) has industrial and medical applications and is used in the treatment of several diseases. Infertility is a medical important issue that its treatment with chemical medicines has various side effects. Due to fewer side effects of herbal medicines, yet little research ...
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Background: Hops (Humulus lupulus L.) has industrial and medical applications and is used in the treatment of several diseases. Infertility is a medical important issue that its treatment with chemical medicines has various side effects. Due to fewer side effects of herbal medicines, yet little research has been done on the hops. Therefore, this study aimed to evaluate the effect of hops extract on sexual hormone levels and sexual dynastic cells in Syrian adult male mice.
Materials and Methods: This experimental study was performed on 40 Syrian adult male mice that were divided into 5 groups of 8: two controls groups and three experimental groups receiving various doses of hops extract (50, 100 and 150 mg/kg). Administrations were done by gavage for 35 days. At the end of the treatment period, blood samples were taken from the heart of animals and testosterone, estrogen and progesterone levels was measured. Also, after isolation of mouse testis, the number of spermatogonia, spermatocytes and spermatid were counted. Data were analyzed using ANOVA and Tukey statistical tests.
Results: The results showed that hop caused a significant increase in estrogen and testosterone levels and spermatogonia and spermatocytes cells number; but has no effect on progesterone levels.
Conclusion: Hops extract, possibly by having phytoestrogen compounds and by stimulating LH secretion, increases estrogen and testosterone levels, and spermatogonia, spermatocytes and spermatid cells number. Therefore, further investigation on hops can utilize to help infertile men.
M ISAPOUR; AR HAKEMI; K EGHBALI; N DAVAR YARI
Volume 15, Issue 1 , March and April 2008, , Pages 20-25
Abstract
Background and Purpose: Clomiphene is the most common drug for the male idiopathic infertility treatment with different results and no valuable factor ha been suggested for prediction of treatment. Therefore the present study was designed to investigate the relationship between early morning total Serum ...
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Background and Purpose: Clomiphene is the most common drug for the male idiopathic infertility treatment with different results and no valuable factor ha been suggested for prediction of treatment. Therefore the present study was designed to investigate the relationship between early morning total Serum Testosterone Level (STL) before treatment and post-clomiphene changes in the spermogram.
Methods and Materials: This clinical trial study was conducted on 172 idiopathic infertile men from 2001 to 2006 in Mashad Iran. Demographic and spermogram data were recorded via questionnaires and patients were divided into 6 groups by their pre-treatment STL. Spermogram parameter changes before and after clomiphene therapy were analysed with SPSS software using t-test ANOVA and Fisher’s test.
Results: Mean age of patients was 31 years; and mean Serum Testosterone Level was 361.3ng/dl. After prescribing clomiphene recovery was observed in the spermogram of in 125 patients (72.7%) with Total Serum Testosterone Level of 200-500ng/dl (P£0.031).
Conclusion: Early morning Serum Testosterone Level can be used as a criterion for predicting the efficacy of clomiphene in male idiopathic infertility.