Volume 24, Issue 6 , March and April 2018, , Pages 45-51
Abstract
Background & Objectives: Mef2c gene induces slow twitch fibers in skeletal muscles which involves in various cellular processes. Endurance activity effects on expression of this genein skeletal muscle. The aim of this study was to investigate the effect of a course of endurance activity on Mef2c ...
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Background & Objectives: Mef2c gene induces slow twitch fibers in skeletal muscles which involves in various cellular processes. Endurance activity effects on expression of this genein skeletal muscle. The aim of this study was to investigate the effect of a course of endurance activity on Mef2c expression in cardiac .
Materials & Methods: In this experimental research, 14 rats under controlled conditions (temperature, light/dark (12:12) cycle, with ad Libitum access to food and water) were housed and randomly were divided into control and experimental groups after familiarization to endurance activity protocol. The experimental group performed 14 weeks endurance activity on motorized treadmill. They were anesthetized and sacrificed after 48 hours from the end of the last session of endurance activity. The heart and left ventricle of rats were removed. Using real time PCR, the expression levels of Mef2c gene was determined. The data were analyzed by t-test.
Results: Endurance activity induces the increase of Mef2c gene expression in heart but it was not significant (p= 0.148) in experimental group compared with control group.
Conclusion: The endurance training possibly does not effect on induction of slow twitch and increasing of them in heart muscle. Therefore, there are unknown mechanisms attempt that the rate of Mef2c gene expression do not much change, to maintain optimal conditions of heart tissue.
Volume 24, Issue 4 , July and August 2017, , Pages 225-231
Abstract
Background & Objective: Med 13 transcriptional factor effects on skeletal muscle response related to stimuli inducing muscular adaptation. The aim of this research was to study the effects of an endurance activity program on med 13 gene expression in slow and fast twitch skeletal muscles.
Materials ...
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Background & Objective: Med 13 transcriptional factor effects on skeletal muscle response related to stimuli inducing muscular adaptation. The aim of this research was to study the effects of an endurance activity program on med 13 gene expression in slow and fast twitch skeletal muscles.
Materials and Methods: The subjects of this study were 14 wistar rats which were housed four weeks under controlled conditions before of start of protocol. Then they were randomly assigned to control and experimental groups. The experimental group performed an endurance activity program (fourteen weeks) on motorized treadmill. They were anesthetized and sacrificed 48 hours after the end of the last session of endurance activity program. The soleus and extensor digitorum longus muscles were removed. Using real time RT-PCR method, the rate of expression levels of med 13 gene determined. Finally, by using t-test, the collected data were evaluated.
Results: The results of this research showed, physical activity significantly increase the expression of med 13 gene in soleus muscle but has no effect on expression of this gene in extensor digitorum longus muscles.
Conclusions: Despite the same endurance activity (duration and intensity), the rate of med 13 gene expression in fast and slow twitch muscles was difference which possibly return to difference of these muscles sensitivity to thyroid hormone.