Traditional medicine
bahareh amin; LEILA aldaghi; hamideh moalemzadeh; fatemeh soltani
Volume 28, Issue 2 , May and June 2021, , Pages 207-213
Abstract
Introduction: Breast cancer is the second common cancer in women at worldwide. Furthermore, patients with breast cancer generally use complementary and integrative medicine such as homeopathy. It has been shown that homeopathic remedies such as Sepia, Phosphorus and Pulsatilla have great effects ...
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Introduction: Breast cancer is the second common cancer in women at worldwide. Furthermore, patients with breast cancer generally use complementary and integrative medicine such as homeopathy. It has been shown that homeopathic remedies such as Sepia, Phosphorus and Pulsatilla have great effects in the various diseases such as some of the cancers. However, the cytotoxicity effects of these compounds have not been studied for breast cancer cell lines such asMCF-7. Hence, the aim of this study was to investigate the cytotoxic effects of these homeopathic remedies on breast cancer cell line. Materials & Methods: Breast cancer cells (MCF-7) were cultured in DMEM medium and treated with different potencies (30 and 200) of remedies for 24, 48 and 72 h. Cell viability was determined by MTT assay. Results: The findings indicate that none of the tested compounds had cytotoxicity effect on MCF-7 cancer cells. Conclusion: This study shows that Sepia, Phosphorus and Pulsatilla as the three of homeopathic remedies have not in vitro cytotoxic effects on breast cancer cell line, and it seems that further studies on different cell lines and also in vivo studies are needed to confirm these findings.
Physical Education
Bentol Hoda asadi; mandana gholami; Hossein Abed Natanzi; nader shakeri
Volume 27, Issue 5 , November and December 2020, , Pages 664-671
Abstract
Introduction: Irisin is one of the skeletal muscle secreted myokine that can affect the growth of cancer cells and influence the muscular tissue changes. In the present study, the effect of four weeks continuous and interval exercise training on serum and muscular levels of irisin in breast cancer bearing ...
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Introduction: Irisin is one of the skeletal muscle secreted myokine that can affect the growth of cancer cells and influence the muscular tissue changes. In the present study, the effect of four weeks continuous and interval exercise training on serum and muscular levels of irisin in breast cancer bearing mice were investigated.
Materials and Methods: The 32 female Balb/c mice assigned in four group with eight mice including: healthy control, tumor control, interval training+tumor and continuous training+tumor groups. In order to induce breast cancer, MC4-L2 cell line were used. Interval and continuous exercise training program conducted in four weeks and five sessions per week. The 48 hours after last exercise training session, blood samples and gastrocnemius muscle tissue were isolated and used for measurement of irisin levels by ELISA method. Data analyze performed by one-way analysis of variance test and Tukey post-hock test and significant considered at p≤0.05.
Results: Significant increase of irisin serum levels in continuous (p=0.002) and interval (p=0.011) training group compared to tumor control group was observed. Tumor volume significantly decreased in continuous and interval training group (p>0.05), but not significant changes in gastrocnemius muscle weight observed after continuous and interval training (p>0.05).
Conclusion: According to present study findings, the observed antitumor effects of continuous and interval training can partly attribute to increase in irisin levels and there is no significant difference between continuous and interval exercise training
immunology & Biochemistry
Mahboobeh Razmkhah; Parisa Karimzadeh; Fatemeh Eghbali; Somayeh Rezaeifard; Zahra Faghih
Volume 25, Issue 6 , November and December 2018, , Pages 809-817
Abstract
Background: Colony stimulating factors (CSFs) are a group of diverse glycoproteins which induce and regulate proliferation and differentiation of hematogenic progenitors in the bone marrow. However, increasing evidence also shows that these factors can also affect and provoke proliferation of non-hematopoietic ...
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Background: Colony stimulating factors (CSFs) are a group of diverse glycoproteins which induce and regulate proliferation and differentiation of hematogenic progenitors in the bone marrow. However, increasing evidence also shows that these factors can also affect and provoke proliferation of non-hematopoietic cells including tumor cells. Therefore, we assessed Granulocyte (G-CSF), Monocyte (M-CSF) and Granulocyte-Monocyte (GM-CSF) colony stimulating factors the serum of breast cancer (BC) and their association with pathological and paraclinical factors of the disease.Materials and Methods: Sixty-two untreated patients with BC as well as 54 age-sex matched controls without any history of cancer and autoimmunity in themselves and their first degree relatives were enrolled. After assigning a consent form, 5 milliliters of peripheral blood were obtained and their serums were separated. The levels of growth factors were then checked by cytokine bead array methods. The data were analyzed by SPSS18 and P-values less than 0.05 were considered as significant.Results: The mean expression of G-CSF, M-CSF and GM-CSF was measured to be 14.18 ± 13.61, 6.11 ± 5.62 and 63.48 ±83.22 in the serum of BC patients, respectively. However, there was no significant difference between patients and controls (P>0.05), further analysis revealed that with increase in the stage of disease from I to III, the serum level of GM-CSF significantly elevated (P=0.016).Conclusion: The results collectively suggest an anti-tumorigenic role for GM-CSF in breast cancer, however, it needs to be confirmed in a more comprehensive studies with more sample size.
genetics
Abolfazl Adli; Solmaz kholdi; Mohammadreza Behroozikhah; Mohammadmehdi Forghanifard
Volume 25, Issue 6 , November and December 2018, , Pages 895-902
Abstract
Introduction: Breast cancer is one of the main cause of death in women in the world. This cancer is heterogeneous and there are three types including LCIS ،(Lobular carcinoma in situ) ،(Ductal carcinoma in situ) DCIS and carcinoma. Although there are chemotherapy and surgery for its treatment, the ...
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Introduction: Breast cancer is one of the main cause of death in women in the world. This cancer is heterogeneous and there are three types including LCIS ،(Lobular carcinoma in situ) ،(Ductal carcinoma in situ) DCIS and carcinoma. Although there are chemotherapy and surgery for its treatment, the molecular mechanism help to diagnosis in the first stage. In this study we aimed to show the expression of CDX1 and CDX2 in breast cancer.Materials and methods:In this study, total RNA was extracted from 40 tumor and 40 normal tissues using RNA extraction kit. After cDNA synthesis with Takara cDNA synthesis kit, expressional analysis of CDX1 and CDX2 gene was evaluated by Real time PCR techniques.Results:Our data showed the expression of CDX1 and CDX2 gene were decreased in this cancer, 50 and 45 percent, respectively. Also our statistical analysis demonstrated the underexpression of CDX1 is correlated with tumor size.Discussion: To the best our knowledge, this is the first study that shows the expression of these gene were reduced in breast cancer. We suggest that these genes have tumor suppressor role in breast cancer and it require more studies to show the main mechanism of these genes in breast cancer
Psychology
Sajjad Basharpoor; nesa kazemi; Mohammadnabi salehi
Volume 25, Issue 5 , November and December 2018, , Pages 731-739
Abstract
Aim: Breast cancer is the most effective emotional and psychological cancer among women, which causes deep emotional and emotional problems in the patient, considering that death anxiety is one of the important psychological variables in cancer patients and self-differentiation level with person's ability ...
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Aim: Breast cancer is the most effective emotional and psychological cancer among women, which causes deep emotional and emotional problems in the patient, considering that death anxiety is one of the important psychological variables in cancer patients and self-differentiation level with person's ability To reduce anxiety. Therefore, the aim of this study was to evaluate the effectiveness of positive group psychotherapy on self-differentiation and death anxiety in women with breast cancer in Bijar city.Materials and Methods: The method Current study was Experimental to pretest-posttest design with control group. A sample was selected from all women with breast cancer, whose names were recorded in Bijar Health Center until 2013. people 30 Based on criteria for having a history of breast cancer and diagnosis by a specialist, having read and write literacy, being married, were selected. They were randomly divided into experimental and control groups. The instrument used in this study was a standard self-differentiation questionnaire (DSI-R), Death Anxiety Scale (DAS), and a positive group psychotherapy package. The datas were analyzed by SPSS-21 software and descriptive indicators and covariance analysis test. Results: The results of covariance analysis indicated that there was a significant difference between the post-test of two groups in self-differentiation (P< 0.000, F=38/58) and death anxiety (p
Anatomy, Histologyو Embryology
Parisa Kerishchi; sayeh Bidaran
Volume 25, Issue 2 , May and June 2018, , Pages 241-250
Abstract
Introduction: Study of Factors affecting regulation and modulation cytokine balance is particularly important in order to inhibit the growth and spread of breast cancer cells in women. In this study the effect of Allium cepa to inhibit the growth of breast cancer cells in female BALB/c mice who have ...
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Introduction: Study of Factors affecting regulation and modulation cytokine balance is particularly important in order to inhibit the growth and spread of breast cancer cells in women. In this study the effect of Allium cepa to inhibit the growth of breast cancer cells in female BALB/c mice who have been patient by induction of cell line 4T1 were investigated.Materials and Methods: In this study have been used of the mice, BALB /c female with breast cancer by injection of mouse cell line 4T1. In each group: Normal control, morbid without treatment (sham) and the experimental group (at the same time induction, were receiving onion root extract food rations) were randomly 8 mice. Daily after washing, dewatering roots red onion (Allium cepa) and fed oral for once a day each of the mouse in the experimental group .1 ml/100gBW/day. The mice are placed under deep anesthesia7 weeks after Induced cell line 4T1. During the seven weeks the mice were weighed every other day .Tumor volume was measured by caliper following the formation of the tumor until the end of the seventh week. The tumor weighed after removal from the body. Spleen removed and was placed in RPMI-1640 medium containing 10%, FBS and ELISA tests were performed to measure the IFN-γ and IL-4 levels. Data analysis was performed using SPSS software (version 18). Analysis of variance (ANOVA) was used to assess differences between groups and Tukey's test was used in order to determine the significance of differences between groups.
genetics
Sonia Faridi; Narges Zeinal zadeh
Volume 25, Issue 2 , May and June 2018, , Pages 259-269
Abstract
Background and objective: Breast cancer is one of the main causes of death among Iranian women. Human RAD51 protein, play a central role in homologous recombination repair of double-stranded DNA breaks and is essential for maintaining genomic stability. A single nucleotide polymorphism in the 5′-untranslated ...
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Background and objective: Breast cancer is one of the main causes of death among Iranian women. Human RAD51 protein, play a central role in homologous recombination repair of double-stranded DNA breaks and is essential for maintaining genomic stability. A single nucleotide polymorphism in the 5′-untranslated region of RAD51 gene (RAD51 135G˃C) is reported to modulate breast cancer risk. The aim of this study was to find out the relationship of this SNP with breast cancer risk among Iranian Azeri Turkish women.Materials and methods: This case-control study was performed on 127 breast cancer cases and 125 controls. Genomic DNA was extracted and the RAD51 135G > C genotype was determined using a PCR–Restriction Fragment Length Polymorphism (RFLP) based assay and confirmed by sequencing. The results were analyzed statistically. Results: The frequencies of CC, CG and GG genotypes of RAD51 135G˃C were 1.613%, 20.161% and 78.225% in control group and 2.362%, 24.409% and 73.228% in patients, respectively. The results showed no significant differences among patients and controls groups.Conclusion: The data presented here may suggest that the RAD51 135G > C polymorphism is not associated with breast cancer risk in Iranian Azeri population.
Mahta Mazaheri Naeeni; Tayebeh Rabbani
Volume 24, Issue 1 , March and April 2017, , Pages 9-15
Abstract
Background & Objectives: Breast cancer is one of the most common cancers in the world that is affected by various genetics, epigenetic and many other environmental factors. Estrogen is one of the risk factors for this cancer. This factor lead to genetic alterations and the beginning and promotion ...
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Background & Objectives: Breast cancer is one of the most common cancers in the world that is affected by various genetics, epigenetic and many other environmental factors. Estrogen is one of the risk factors for this cancer. This factor lead to genetic alterations and the beginning and promotion of breast cancer. Materials & Methods: In this review, we provide information using databases of NCBI, PubMed, Google Scholar, and Ovid MEDLINE, about the molecular basis of breast cancer, the effect of estrogen hormone, and estrogen receptors on cancer incidence, the use of anti-estrogens such as Tamoxifen in treatment of breast cancer and mechanisms of resistant to these drugs. Result: Anti-estrogens such as tamoxifen play an important role in treatment of estrogen-receptor positive breast cancers by preventing estrogen binding to its receptors in these tumors. Finding the molecular basis of breast cancer will help us to achieve effective treatment for breast cancer. Conclusion: Increased estrogen and estrogen receptor highly influence the incidence of breast cancer. Tamoxifen is standard adjuvant therapy for women with ER-positive [+], but there is some intrinsic or acquired resistance to endocrine treatment that require further investigations.
Ahmad Hamta; Narges Sharif
Abstract
Backgrounds Breast cancer is the most common cancer in females worldwide. Phosphatidylinositol 3-kinases (PI3Ks) evolve from lipid kinase that regulates the diverse cellular signaling pathways and are often altered in human cancers. Mutations in the gene encoding the p110α catalytic subunit PI3K ...
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Backgrounds Breast cancer is the most common cancer in females worldwide. Phosphatidylinositol 3-kinases (PI3Ks) evolve from lipid kinase that regulates the diverse cellular signaling pathways and are often altered in human cancers. Mutations in the gene encoding the p110α catalytic subunit PI3K (PIK3CA) can increase the enzyme activity and cause uncontrolled growth in cells. Previous studies indicated high frequency of mutation in PIK3CA gene in breast cancer. The current study aimed at determining the activating mutations of PI3K gene that can treat breast cancer using kinase activity of the enzyme inhibitors.Methods & Materials In the current study, due to the high rate of breast cancer in Markazi province, mutations of PIK3CA gene in 45 patient samples and 20 controls were investigated by the means of single-strand conformational polymorphism (SSCP) and direct DNA sequencing. In addition, the correlation between PIK3CA mutations and clinicopathological factors including age at diagnosis, lymph node metastases, subtype histology, tumor size, and histological grade were investigated by the Pearson Chisquare (X2) test.Results Among the 26.6% PIK3CA gene mutations in the study, 75% were identified in the exon 9; and accordingly, in addition to hotspot mutation [G1624A (E542K)], another mutation [G1634C (E545A)] was also detected. The current study showed no significant correlation between PIK3CA mutations and clinicopathological factors. Conclusion Mutations in PI3K gene, a proto-oncogene, showed the importance of this pathway for therapeutic purposes to prevent and cease the growth of breast cancer
Leila Saadat Aldaghi; Hasan Rezaee Seresht; Hamid Cheshomi
Volume 23, Issue 2 , March and April 2016, , Pages 353-359
Abstract
Background & Objectives: Breast cancer is the most common cancer affecting women so that it is the second common cancer (after lung cancer) in women. Vitex pseudo negundo is used as a traditional medicine. Recently, the biological activities of Vitex pseudo negundo plants have been reported as possessing ...
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Background & Objectives: Breast cancer is the most common cancer affecting women so that it is the second common cancer (after lung cancer) in women. Vitex pseudo negundo is used as a traditional medicine. Recently, the biological activities of Vitex pseudo negundo plants have been reported as possessing anticancer, antibacterial, antiulcer and antifungal properties. However, the antitumor effects of this medicine have not been studied in cancer cell lines. The aim of this study was to investigate the antitumor effect of Vitex pseudo negundo fruit on breast cancer cell lines. Materials & Methods: Breast cancer (MCF7) cell lines were cultured in DMEM medium. Vitex pseudo negundo fruit was extracted; and different dilutions of Vitex pseudo negundo extract (5mg/mL to 100mg/mL) were added to cell culture. Cell viability was quantitated by MTT assay after 72 hours. Results: The findings indicate that the extract of Vitex pseudo negundo fruit on MCF7 cancer cell lines had cytotoxicity in all concentrations and the highest inhibition was 50 and 100 mg/ml concentrations. Conclusion: Our study shows that Vitex pseudo negundo fruit extract has cytotoxic effects on tumor cells, It seems that Vitex pseudo negundo fruit could be considered as a promising chemotherapeutic agent in cancer treatment.
Akram Kooshki; Roya Akbarzadeh; Fatemeh Ghardashi; Masoumeh Hashemian; Raha Salehabadi; Manidheh Yousefi Moghaddam; Mohsen Hiteh
Volume 22, Issue 5 , November and December 2015, , Pages 716-723
Abstract
Background& Objectives: Breast cancer is one of the most common cancers in the women and, since nutrition is one of the factors in thise disease, the present study was conducted to assess the effect of energy, macro nutrients and antioxidants intake in women on the breast cancer in Sabzevar. Materials ...
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Background& Objectives: Breast cancer is one of the most common cancers in the women and, since nutrition is one of the factors in thise disease, the present study was conducted to assess the effect of energy, macro nutrients and antioxidants intake in women on the breast cancer in Sabzevar. Materials & Methods: This cross-sectional study was conducted on 125woman with breast cancer in Sabzevarin 2011. Census sampling was done in homes by volunteers and referral of suspected women to mammography, ultra sound and radiology clinicand, also, and Sabzevar center of cancer statisticsforexaminationand diagnosis of breast cancer. After writting informed consent by patients, a demographic questionnaire and semi-quantitative frequency of 160 foods wascompleted by the trained interviewers. The obtained nutritional information was analysedusing the Software Nutritioni IV. Descriptive statistics, frequency, one-sample t-test and non-parametric X2 tests were assessed using SPSS 16 software, and PResults: The mean BMI of patients was 22.5±5.7 kg/m2, the average age of women was 50.7 ± 1years, mean of the first menstrual period was 13.2±1.6 years and mean of menopause 46.4±4.7years. Dietary assessment showed mean of energy, protein, fat and carbohydrate intakes were 2309±1389.24, 64.26±19.05, 65.84±21.70 and 363.79± 154.72 ,respectively (P<0.05). Also, in this study, energy and macronutrients intakeswere rather than dietary standard values, andantioxidants of vitamin A, E, C and selenium intake were less than dietary standard values in women. Conclusion: The findings showed that energy and macronutrients intakesrather than dietary standard values, and antioxidants intake was less than dietary standard values in women.
Mostafa EnayatRad; Hamid Salehinia
Volume 22, Issue 1 , March and April 2015, , Pages 27-35
Abstract
Background and purpose: Breast cancer is the commonest cancer and first leading cause of death among women. Since having knowledge about breast cancer incidence trends over time is essential for health planning, this study was aimed at investigating variation in the incidence of breast cancer.
Materials ...
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Background and purpose: Breast cancer is the commonest cancer and first leading cause of death among women. Since having knowledge about breast cancer incidence trends over time is essential for health planning, this study was aimed at investigating variation in the incidence of breast cancer.
Materials and Methods: This study is a reanalysis of existing data published by cancer registry system of Iran during 2003 to 2009. The reported incidence of WHO standards are based on the direct method.
Results: According to registry statistics between 2003 and 2009 breast cancer incidence among women has increased as 3946 and 8424 cases were recorded in years 2003 and 8424 respectively. . Over mentioned years the number of breast cancer cases recorded in the country was 45122, in which 43922 cases have occurred among women. The Highest ASR was reported about 33/21 in year 2008 and the lowest was reported about 15/96 in year 2003 (cases per hundred thousand people) ,The highest and lowest recorded incidence rates appertain to Tehran and Kohkiloye and Boyer Ahmad. Cancer registry statistics during 2003 to 2009 indicate that cancer incidence peak age is around 50-55 years or more.
Conclusions: Breast cancer incidence rate has been increasing in recent years, as the most common malignancy among women in Iran. The incidence of the disease is rising considerably in the country, especially in the central and Northern provinces; therefore, planning for early detection of cancer in high-risk areas would be useful.
Roya Akbarzadeh; Fatemeh Ghardashi; Rasool Tabari; Akram Koshki; Masoumeh Hashemiyan; Tayyebeh Novrozinejad; Hamid Alami
Volume 20, Issue 5 , March and April 2014, , Pages 582-589
Abstract
Title: Evaluation of individual characteristics, family history and blood group in women with breast cancer in Sabzevar, 1391
Background: Breast cancer is the most common cancer in women globally. This disease has varied Incidence and mortality rate in different communities which this variation is affected ...
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Title: Evaluation of individual characteristics, family history and blood group in women with breast cancer in Sabzevar, 1391
Background: Breast cancer is the most common cancer in women globally. This disease has varied Incidence and mortality rate in different communities which this variation is affected by some factors such as age, sex , race ,socioeconomic and marital status, geographic location and lifestyle. this study aimed to examine individual characteristics, family history and blood group in women with breast cancer in Sabzevar.
Materials and Methods: In this cross-sectional survey, 218 women with age of older than 20 years suffering from breast cancer were identified through census method by health volunteers from 1380 to 1389 in Sabzevar. Needed information was collected using an interview form whose validity and reliability were approved via face validity and content and test-retest method. Obtained data was analyzed using spss software and descriptive statistics was applied to calculate indexes and frequencies.
Result: among 218 patients, 125( 57.3 percent ) were alive . the Subjects’ average age was 52.85± 1.2 with a Maximum and minimum age of 86 and 28 years old, respectively. among 124 live patients , 26 patients ( 21 %) of them had a history of breast cancer in the family. If there is a first-degree relatives with breast cancer, chance of getting cancer would be higher than other cases ( 46.1 %). The most and least frequency of blood group were O (37.6 %) and AB + (6.9 percent), respectively.
Conclusion: findings of the present study revealed that the family history and blood group of O can be considered as risk factors of developing breast cancer in Sabzevar.The mean age of onset is lower than the worldwide age. Enhance, these issues should be noticed in the planning of health services.
Tayyebeh Masoomi; Narges Shafaroudi; Afsoun Hasani Mehraban; Mohammad Kamali; Ramesh Omrani pour
Volume 20, Issue 1 , March and April 2013, , Pages 84-92
Abstract
Background: Breast cancer has high incidence among Iranian women and their long term survival lead to have more challenges with cancer complication. This qualitative study aimed to identify psychological responses following breast cancer. Materials and methods: A qualitative phenomenological method ...
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Background: Breast cancer has high incidence among Iranian women and their long term survival lead to have more challenges with cancer complication. This qualitative study aimed to identify psychological responses following breast cancer. Materials and methods: A qualitative phenomenological method has been designed for the study. A sample of 11 women with breast cancer by purposive sampling selected and semi-structured in-depth interviews were conducted. The interviews were transcribed verbatim,then Information have been analyzed during 5 steps using smith’s method. Results: Two main themes emerged from the interviews included basic reactions and residual reactions and The following six sub themes of despair of life, trying to find a guilty, social isolation , irritability-sensitivity, feeling incompetence and fear of disease recurrence has been determined. Conclusions: Although breast cancer is a physical disease but it has great impact on a person's mental health. Also So many psychological problems caused by breast cancer and its treatment, remains until many years after completion of therapies.