Fatemeh Zihreh; Siam Nasri; Parisa Karishchi
Volume 22, Issue 2 , May and June 2015, , Pages 377-386
Abstract
Background and purpose: Antioxidants such as Quercetin protect sperm cells from free radical damage. This research was conducted to investigate the effects of Quercetin on spermatogenesis in male rats.
Materials and Methods: In this experimental study 50 male rats were used. Quercetin was injected daily ...
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Background and purpose: Antioxidants such as Quercetin protect sperm cells from free radical damage. This research was conducted to investigate the effects of Quercetin on spermatogenesis in male rats.
Materials and Methods: In this experimental study 50 male rats were used. Quercetin was injected daily at doses of 5, 10, 15, 20 (mg/kg) for two weeks intraperitoneally. One week after the last injection, blood samples were collected and the left testis of rats were removed and weighed. Epididymal sperm and testicular sections were also studied. Data were analyzed by using one-way ANOVA at the significant level of 0.05 in SPSS 19 software.
Results: No significant difference was found in serum level of gonadotropins, but testosterone has increased at dose of 20 mg/kg (6.52±0.50, P=0/0079).Significant difference in testicular weight wasn’t seen. The number of sperm (51.35±1.68, P=0.0251) and also sperm mobility (73.88±5.91, P= 0.0002) in the epididymis especially at dose of 20 mg/kg has increased. No significant difference was found between the groups in the number of spermatogonia cells. But the number of primary spermatocytes (118.25±7.04, P=0.0171), spermatids (106.75±6.28, P=0.0032) and Spermatozoa (101.75±6.30, P= 0.0008) were increased at dose of 20 mg/kg.
Conclusion: No significant change in gonadotropins was seen, which indicates that probably Quercetin has not affected PG axis. Quercetin is believed to improve the quality and quantity of germ cells with its antioxidant effects and increasing of testosterone.
Zeynab Amiri; Mehrdad Modaresi
Volume 18, Issue 1 , March and April 2011, , Pages 13-20
Abstract
Background and Purpose: Oxcarbazepine is a widely used novel antiepileptic drug. In vitro electrophysiologic studies indicate that oxcarbazepine can block voltage-sensitive sodium channels، enhance the functioning of the neural membranes، and inhibit the propagation of synaptic impulses. It is also ...
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Background and Purpose: Oxcarbazepine is a widely used novel antiepileptic drug. In vitro electrophysiologic studies indicate that oxcarbazepine can block voltage-sensitive sodium channels، enhance the functioning of the neural membranes، and inhibit the propagation of synaptic impulses. It is also necessary to investigate the effect of oxcarbazepine on sex hormones because of its effects on the nervous system and the close neuro-hormonal association of this system with reproduction. In this research، the effect of oxcarbazepine was studied on the amount LH، FSH، testosterone Hormones and male reproductive physiology.
Methods and Materials: This quasi-experimental research involved 40 adult male volunteers aging from 25 to 45 years old. They suffered from epilepsy and were selected from those admitted to the neurological clinics of Shiraz، Iran، and were assigned into four groups of 10: Control group receiving no materials، and experimental groups receiving 150، 300، and 600 mg/day oxcarbazepine as oral tablets for 30 days. The study was conducted upon the permit of the ethics committee and after the informed consent was signed by the participants. After the termination of this period، the levels of LH، FSH، and testosterone Hormones in the blood were measured. The obtained data were analyzed in SPSS 11.5 using Tukey، Duncan tests and ANOVA.
Results: According to the findings، plasma concentration of LH showed a significant increase (p
Fakher Rahim; Ghasem Saki; Majid Jasemi
Volume 16, Issue 1 , March and April 2009, , Pages 6-11
Abstract
Background and Purpose: Sperm count and their productivity are dependent on the levels of blood androgenic hormones. Also it is likely that the Centella asiatica extract can affect the levels of hormones affecting spermatogenesis. Therefore this study was conducted to determine the effect of Centella ...
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Background and Purpose: Sperm count and their productivity are dependent on the levels of blood androgenic hormones. Also it is likely that the Centella asiatica extract can affect the levels of hormones affecting spermatogenesis. Therefore this study was conducted to determine the effect of Centella asiatica extract on serum levels of testosterone FSH and LH in male Wistar rat. Methods and Materials: In this experimental study 40 adult male albino Wistar rats were divided into 5 groups: an untreated control sham receiving a solvent medium and the remaining three groups (experimental groups 1 to 3) which received 10 mgkg-1 50 mgkg-1 and 80 mgkg-1 Centella asiatica extract every day for 40 days. Twenty four hours after the last administration of extract 3-4 ml of blood samples were collected from every rat by aspiration from heart ventricle and their serum concentrations of FSH LH and testosterone were measured using radioimmunoassay method. The obtained data were analyzed in SPSS13 using one-way ANOVA and Tukey test. Results: The mean and standard deviation of testosterone the control and sham groups were 16.4±2.18 and 14.1±0.09 respectively; in the experimental groups (10 50 and 80 mg/kg) these were 15±1.32 9.8±0.05 and 8.4±0.31 nanoM/l respectively. Mean testosterone level in rats receiving 50 mgkg-1 and 80 mgkg-1 Centella asiatica extract were significantly lower than the control groups (p=0.001 P=0.008) and sham groups (p=0.001 P=0.003); also it reduced in comparison with the experimental group receiving 10 mgkg-1 Centella asiatica extract (p=0.02 P=0.004). The difference between the two groups receiving 50 and 80 mgkg-1 Centella asiatica extract was not significant (p=0.09). The concentration of FSH and LH in all experimental groups was not significantly different from both control and sham groups (p>0.05). Conclusion: By affecting Leydig cells and causing disorder in the levels of testosterone sperm count and by affecting epididymis centella Asiatica alcoholic extract can reduce the motility and productivity of sperms.