Document Type : Original Article
Authors
1 Master of Sciences in Microbial Biotechnology, Department of Biology, Payam Noor University, Iran
2 Associated Professor, Department of Biology, Payam Noor University, Iran
3 Professor, Department of Biochemistry, Payam Noor University, Iran
Abstract
Background: Microbial lipases are an important group of enzymes with biotechnology value. In the present research, an attempt was made to isolate and identify lipase-producing microbial strains from industrial wastewater samples.
Materials and Methods: After taking samples from sewage and sewage from different places,16 colonies were isolated from these samples. The isolates were cultured in a specific culture medium containing Tween80 to check the ability to produce lipase enzyme. Enzyme activity was determined using the light absorption curve. In order to identify the isolates molecularly, ribotyping was performed. For this purpose, the DNA of the isolates was extracted and PCR was performed with the help of 16SrRNA gene primers. The PCR product was sequenced and the strains were identified using sequence blast in the NCBI database.
Results: Out of a total of 16 isolates, ten strains (62.5%) were able to produce lipase enzyme as a result of creating a transparent halo in the culture medium of the lipid test. Among these, two isolates with the same halo formation rate and source of isolation, which had the highest growth and activity after 144 hours were selected from the culture. Enzyme activity values for bacteria isolated from slaughterhouse effluent and garage effluent ranged from 2.99 to 22.65 and 3.73to 39.2 units/ml, respectively.
Conclusion: Due to their very high lipase activity compared to the strains introduced in other researches, Aeromonas veroni and Copriavidus metallidurans bacteria are suggested as very suitable and efficient strains for the biological treatment of wastewater.
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Main Subjects
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