Bio-control of  Acinetobacter baumannii by Bdellovibrio as a predatory bacteria

Jafarian, Neda; Akhavan Sepahi, Abbas; Naghavi, Nafiseh Sadat; Hosseini, Farzaneh; Nowroozi, Jamileh

Document Type : Original Article

Authors

¹ Ph.D Student of Microbiology, Department of Microbiology, School of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran

² Associate professor, Department of Microbiology, School of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran

³ Assistant professor, Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

⁴ Assistant professor, Department of Microbiology, School of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran

⁵ Professor, Department of Microbiology, School of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran

Abstract

Introduction: Management of antimicrobial-resistant Acinetobacter baumanni is a great challenge for clinical microbiologist. Whereas use the predatory bacteria is the best way to treat infection diseases caused by antibiotic-resistant bacteria, the aim of this study was to use the autochthonous Bdellovibrio potential to prey Acinetobacter baumannii as a biological control agent.
Materials and Methods: To evaluate the effect of autochthonous Bdellovibrio on Acinetobacter baumannii, as a biological control agent, plaque perdition assay, reduction in optical density (OD) and reduction in host cells viability by colony forming unit (CFU) counting in cocultures after 0,24, 48 hours and assay of killing efficiency were carried out.
Results: Clear plaques were observed after 3-6 days of incubation. In co-cultures, the CFU enumeration of Acinetobacter baumannii was decreased after 48 hours. Also, after 48 hours, OD was decreased 0.7unit. In this research the efficiency ofBdellovibriokilling for Acinetobacter baumannii was 83%.
Conclusion: Base on the results, Bdellovibrio can prey Acinetobacter baumannii as a prey cell. Therefore utilize of Bdellovibrio spp., as biological control agent, for treatment of antimicrobial-resistant Acinetobacter baumannii infection suggested in future study.

Keywords

Main Subjects

Microbiology

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Journal of Sabzevar University of Medical Sciences

Bio-control of Acinetobacter baumannii by Bdellovibrio as a predatory bacteria

References

References

Volume 27, Issue 5
November and December 2020
Pages 622-628

Bio-control of Acinetobacter baumannii by Bdellovibrio as a predatory bacteria

References

References

Volume 27, Issue 5November and December 2020Pages 622-628

Volume 27, Issue 5
November and December 2020
Pages 622-628