Biotechnology & nanotechnology
Mahta Majdnia; Maryam Sadrnia; Fatemeh Shahbazi; Nooshin Sohrabi
Abstract
Introduction: One of the best ways to remove toxic metals is to use bacteria resistant to these metals with biological purification process. The aim of this research is to isolate bacteria resistant to tin, copper, chromium and nickel from industrial wastewater and their molecular identification.
Materials ...
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Introduction: One of the best ways to remove toxic metals is to use bacteria resistant to these metals with biological purification process. The aim of this research is to isolate bacteria resistant to tin, copper, chromium and nickel from industrial wastewater and their molecular identification.
Materials and methods: First, wastewater contaminated with heavy metals was collected from electroplating factories located in Tehran province. The wastewater sample was cultured on LB Agar containing certain concentrations of heavy metals and the grown bacteria were isolated. On the grown bacteria, the minimum growth inhibitory concentration (MIC) of heavy metals was determined by the microbroth dilution method. Genomic DNA of two strains with the highest level of resistance, purity and polymerase chain reaction was performed with the help of specific primers. The PCR product was sequenced and ribotyping was done.
Results: 9 gram positive and negative bacilli and gram negative coccobacilli were isolated from wastewater containing heavy metals. Two Gram-negative bacillus strains showed the highest resistance to heavy metals in the MIC test. Based on the sequencing results, these two strains were identified as Enterobacter and Pseudomonas.
Conclusion: Two strains with the highest resistance to four metals were isolated from the effluent of electroplating factories and phylogenetic evaluation was performed. These bacteria can be used in the biological treatment of wastewater containing heavy metals.
Biotechnology & nanotechnology
Naimeh Khazali; Maryam Sadrnia; Reza Hajihosseini
Abstract
Background: Microbial lipases are an important group of enzymes with biotechnology value. In the present research, an attempt was made to isolate and identify lipase-producing microbial strains from industrial wastewater samples.Materials and Methods: After taking samples from sewage and sewage from ...
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Background: Microbial lipases are an important group of enzymes with biotechnology value. In the present research, an attempt was made to isolate and identify lipase-producing microbial strains from industrial wastewater samples.Materials and Methods: After taking samples from sewage and sewage from different places,16 colonies were isolated from these samples. The isolates were cultured in a specific culture medium containing Tween80 to check the ability to produce lipase enzyme. Enzyme activity was determined using the light absorption curve. In order to identify the isolates molecularly, ribotyping was performed. For this purpose, the DNA of the isolates was extracted and PCR was performed with the help of 16SrRNA gene primers. The PCR product was sequenced and the strains were identified using sequence blast in the NCBI database.Results: Out of a total of 16 isolates, ten strains (62.5%) were able to produce lipase enzyme as a result of creating a transparent halo in the culture medium of the lipid test. Among these, two isolates with the same halo formation rate and source of isolation, which had the highest growth and activity after 144 hours were selected from the culture. Enzyme activity values for bacteria isolated from slaughterhouse effluent and garage effluent ranged from 2.99 to 22.65 and 3.73to 39.2 units/ml, respectively.Conclusion: Due to their very high lipase activity compared to the strains introduced in other researches, Aeromonas veroni and Copriavidus metallidurans bacteria are suggested as very suitable and efficient strains for the biological treatment of wastewater.
Biotechnology & nanotechnology
Fadhl Rajeh Swade; farahnaz molavi; samaneh dolatabadi
Volume 29, Issue 3 , September and October 2022, , Pages 421-434
Abstract
Introduction: At present, Pseudomonas aeruginosa bacteria has caused many problems to control nosocomial infections due to its resistance to various antibiotics and MexAB-OprM efflux pumps play an important role in making Pseudomonas aeruginosa strains resistant to antibiotics. The aim of this study ...
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Introduction: At present, Pseudomonas aeruginosa bacteria has caused many problems to control nosocomial infections due to its resistance to various antibiotics and MexAB-OprM efflux pumps play an important role in making Pseudomonas aeruginosa strains resistant to antibiotics. The aim of this study was to determine the antimicrobial effect of silver nanoparticles and probiotic Lactobacillus plantarum on MexA gene expression.
Materials and Methods: In this descriptive cross-sectional study, 49 strains were collected from 8 medical diagnostic laboratories in Mashhad during the years 1398-1399 for 8 months. Evaluation of antibiotic susceptibility pattern of strains was using disk diffusion method based on CLSI protocol. After treatment of multidrug resistant bacteria with inhibitors, microdilution method and Real time-PCR technique were used to determine the effective dilution of silver nanoparticles and probiotics on the expression of MexA gene of its producing bacterium, respectively.
Results: All samples were identified and all of them were resistant to more than two antibiotics and had MexA gene. The minimum growth inhibition concentration in agar dilution method was up to 250 μg / ml for silver nanoparticles and up to 18 μg / ml for probiotics. Silver nanoparticles had a greater effect in inhibiting bacterial growth compared to probiotics and this effect is less than the combined effect of probiotics and silver nanoparticles (P> 0.05).
Conclusion: Silver and probiotic nanoparticles have inhibitory and antibacterial activity to reduce the performance of MexAB-OprM efflux pump in Pseudomonas aeruginosa. (P> 0.05).
Biotechnology & nanotechnology
Ali Ghorbani Ranjbary; Jalil Mehrzad; Hesam Dehghani; Abbas Abdollahi; Saman Hosseinkhani
Volume 29, Issue 1 , May and June 2022, , Pages 141-153
Abstract
Background Colorectal cancer (CRC) is one of the most common cancers in the world. The role of IL-17A in cancer begins in the early stages of tumorigenesis and appears to play an important role in tumorigenesis by causing inflammation. The present study was conducted to investigate the expression of ...
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Background Colorectal cancer (CRC) is one of the most common cancers in the world. The role of IL-17A in cancer begins in the early stages of tumorigenesis and appears to play an important role in tumorigenesis by causing inflammation. The present study was conducted to investigate the expression of KI67 and P53 genes in HT-29 colon epithelial cells with IL-17A.Materials and Methods IL-17A was purchased and 50 ng/ml was added to HT-29 culture medium and after 24 hours the cells were isolated from the culture medium and cell necrosis was examined by MTT. Then RNA was extracted and the expression levels of P53 and KI67 were analyzed using newly designed primers by Reverse transcription (RT) qPCR method and GeniX6 software.Results The MTT test showed that a concentration of 150 ng/ml for 24 hours had maximal necrosis rate in HT-29. After 24 hours of IL-17A incubation, the expression of KI67 (P = 0.003) and P53 (P = 0.001) genes in HT-29 cells in the IL-17A exposed group decreased and increased, respectively. Also, compared to the control group the number of examined HT-29 cells in the IL-17A treated group showed a significant decrease (P
Biotechnology & nanotechnology
Nakisa Zarrabi Ahrabi; Seyed Mehdi Tabaie; MARYAM JAHANSHIRI
Volume 28, Issue 5 , November and December 2021, , Pages 663-674
Abstract
Introduction: The use of nanoparticles in biomedical research have been increasingly developed in recent years. One of the applications of nanotechnology is the use of compounds such as hydrogels, micelles, nanoliposomes, nanofibers and niosomes as a delivery system to treat cancer. The aim of this study ...
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Introduction: The use of nanoparticles in biomedical research have been increasingly developed in recent years. One of the applications of nanotechnology is the use of compounds such as hydrogels, micelles, nanoliposomes, nanofibers and niosomes as a delivery system to treat cancer. The aim of this study was to evaluate the ant antitumor effect of the caffeine-loaded niosomes on MCF-7 human breast cancer cell line.Materials and Methods: The thin film hydrate on method was used to prepare caffeine-loaded niosomes. Certain amounts of surfactant, caffeine and cholesterol were dissolved in ethanol. Rotary evaporation was used to remove solvent from reaction mixture. Sample was dissolved in the phosphate buffer and homogenized with sonicator. The average diameters of caffeine-loaded niosomes were measured using a Zetasizer Nano system. Finally, the cytotoxicity effect was evaluated by MTT assay.Results: The treatment of breast cancer cells with different concentrations of nano-sized drug and free drug showed that the nanoparticles had the ability to inhibit the MCF7 proliferation more than free drug. 0.25 mg/ml of niosomal drug had a greater effect on reducing of cancer cells viability.Conclusion: Vesicular nanoparticles are complex systems with some advantages and disadvantages which sets these delivery systems apart from other colloid system. Vesicular nanoparticle is being developed as new drug delivery system for cancer treatment.
Biotechnology & nanotechnology
Ezzat Nourizadeh
Volume 28, Issue 5 , November and December 2021, , Pages 775-789
Abstract
Introduction: Leishmania (L.) infantum is the etiologic cause of visceral leishmaniasis in Iran. Efficient vaccines and diagnosis methods are required to control leishmaniasis. The aim of this study is produce and optimize monoclonal antibodies against promastigotes forms of L. infantum antigen.
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Introduction: Leishmania (L.) infantum is the etiologic cause of visceral leishmaniasis in Iran. Efficient vaccines and diagnosis methods are required to control leishmaniasis. The aim of this study is produce and optimize monoclonal antibodies against promastigotes forms of L. infantum antigen.
Materials and Methods: The mice were vaccinated with the L. infantum antigen and their antibody titers were determined by the ELISA method. Spleen cells of the most immune mouse were fused with SP2/0 in the presence of Poly Ethylene Glycol.The effect of supernatant of SP2/0 and mice peritoneum macrophage cells culture (SSMCC) on hybridoma cell proliferation was studied.
Results: Among the 12 fusion, a total of 26 monoclonal were positive.12 of which had acceptable optical absorbance in OD 450 nm. Finally, 4 clones, designated as 8D2 FVI6, 8D2 FVI3, 6G2 FV4 and 6G2 FV3. From these hybrids, anti-promastigotes L. infantum monoclonal antibodies were obtained. SSMCC was shown to play a key role in hybridoma proliferation and of mAb production. It seemed that SSMCC is rich of growth factors.
Conclusion: It seems in the near future, this SCCSM can be used as a growth factor for cancerous and non-cancerous cells in research centers at a wider level.
Biotechnology & nanotechnology
fatemeh karimiyan; mahboobeh madani; noshin naghsh
Volume 28, Issue 4 , September and October 2021, , Pages 545-555
Abstract
Introduction: Today, carotenoids draw attention because of their antioxidant and antitumor properties and also usage as natural food colors. In this study, the biotechnological production of carotenoid by the yeast Rhodoturola musilaginosa and its effect on the lipid profile of male mouse were investigated.
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Introduction: Today, carotenoids draw attention because of their antioxidant and antitumor properties and also usage as natural food colors. In this study, the biotechnological production of carotenoid by the yeast Rhodoturola musilaginosa and its effect on the lipid profile of male mouse were investigated.
Materials and Methods: In this semi-experimental study, carotenoids were extracted from R. mucilaginosa using the Davis method. Twenty four male mice were randomly divided into four groups of six each. Two treatment groups received 16 and 32 mg/kg carotenoid peritoneally. One group received 400 mg/kg R. mucilaginosa powder by gavage. Control group received 0.5 ml physiological serum intraperitoneally. Blood samples were then collected and lipid profile was studied for triglyceride, cholesterol, LDL and HDL. The results were analyzed by SPSS 21 and comparisons were conducted using one-way ANOVA and Kruskal-Wallis test.
Results: The lowest serum concentrations of triglyceride and VLDL were observed in the group given 32 mg/kg carotenoid compared to the gavage and control groups. Mean serum cholesterol and HDL concentrations in the groups were not significantly different. The highest mean LDL concentration was observed in the gavage group.
Conclusion: According to the results of this study, R. muscilaginosa carotenoid decreased serum concentrations of triglyceride, VLDL and cholesterol. Therefore, it seems that the possibility of using it as a dietary supplement be helpful.
Biotechnology & nanotechnology
Aysan Ghahremani; Ebrahim Ganji_Moghadam; Maryam Tatari; Susan Khosroyar
Volume 28, Issue 2 , May and June 2021, , Pages 274-285
Abstract
Introduction: Paneer-boot, with its antifungal and antibacterial effects, blood sugar and cholesterol-lowering, immune system, and antioxidant activity, has recently received pharmacologists' attention due to its high compatibility. Due to difficult ecological conditions, it can grow in the country's ...
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Introduction: Paneer-boot, with its antifungal and antibacterial effects, blood sugar and cholesterol-lowering, immune system, and antioxidant activity, has recently received pharmacologists' attention due to its high compatibility. Due to difficult ecological conditions, it can grow in the country's tropical regions such as Sistan and Baluchestan Province .
Materials & Methods: This study was designed in a completely randomized design with four replications under laboratory conditions at Khorasan Razavi Agricultural and Natural Resources Research and Education Center. Concentrations of 0, 2, 4, 6, 8, and 10 bar PEG were used to induce the effect of drought stress on MS medium containing BAP on secondary traits of cheese .
Results: The results showed that the drought stress significantly increased antioxidant enzymes such as catalase, peroxidase, ascorbate peroxidase, proline, phenol, and flavonoid content membrane leakage. On the other hand, it resulted in a significant decrease in the relative humidity content, potassium ion content and potassium to sodium ratio. Also, most of the studied traits had a significant correlation. Conclusion: The results showed that the Paneer-boot plant had acceptable tolerance to induction of drought stress with polyethylene glycol. Enzymatic and non-enzymatic mechanisms had a significant role in reducing the adverse effects of drought stress. On the other hand, drought stress increased some metabolites, such as phenol and flavonoids, which have medicinal uses .
Biotechnology & nanotechnology
fereshteh jookar kashi; Zohreh Boroumand
Volume 28, Issue 1 , January and February 2021, , Pages 144-155
Abstract
Introduction: The bacteria with a remarkable ability to regenerate heavy metal ions are considered biological factories or important nano-factories to produce nanoparticles. The bacterial production of nanoparticles as a green and one-step method can overcome production problems and disadvantages through ...
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Introduction: The bacteria with a remarkable ability to regenerate heavy metal ions are considered biological factories or important nano-factories to produce nanoparticles. The bacterial production of nanoparticles as a green and one-step method can overcome production problems and disadvantages through physical and chemical methods. Materials and Methods: In this study, bacteria isolates were taken from soil samples of Nakhlak mine. One silver resistant bacterial strain was selected for the production of silver nanoparticles. This strain synthesized silver nanoparticles with supernatant and biomass. The nanoparticles synthesized were characterized using XRD and SEM. The antimicrobial activity of the nanoparticles was determined. Results: The silver resistance bacteria were isolated from soil samples. One silver resistant bacterial strain was selected for the production of silver nanoparticles. The nanoparticles showed good antimicrobial activity against standard strains of tested microorganisms. Conclusion: The nanoparticle synthesized by bacterial strain isolated from Nakhlak mine is a promising new biological source for synthesizing silver nanoparticles with potent antimicrobial activity. According to the results, this compound can be used to make disinfectants
Biotechnology & nanotechnology
Saeid Mirzaei; Malek Hossein Asadi; Pegah Hejazi; Babak Taghipour
Volume 27, Issue 6 , January and February 2021, , Pages 754-759
Abstract
Introduction: Stomach cancer and breast cancer are most common cancers in the world and in Iran. Today, the use of fungal and bacterial extractions has been interesting to cancer researchers. Rhizobium is a highly beneficial bacteria in agriculture and environment that provides the nitrogen needed for ...
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Introduction: Stomach cancer and breast cancer are most common cancers in the world and in Iran. Today, the use of fungal and bacterial extractions has been interesting to cancer researchers. Rhizobium is a highly beneficial bacteria in agriculture and environment that provides the nitrogen needed for Legume family plants through coexistence with this family. Materials and Methods: In this study, the effect of Bradyrhizobium cell extraction and its mutated type on the cancer of breast (MCF7) and stomach (AGS) was investigated. Material and Methods: Cytotoxicity effect of different concentration of Bradyrhizobium japonicum biomass and extract on stomach and breast cancer cells was investigated using MTT colorimetric method. Data analysis were conducted using Graphpad Prism 6.0 software and ANOVA method. Results: The findings showed that both wild type and mutant type of bacteria had a significant meaningful effect on both types of cancer cells and their cytotoxicity was dependent on the concentration of extract or biomass (the lowest rate was observed at a concentration of 30 and at most 480 Micrograms per ml). In most cases biomass showed a better effect than extract. Conclusion: In general, this study showed the effect of extract and biomass of Bradyrhizobium bacteria cytotoxicity on cancer cells, which could be used as a source for antineoplastic agent.
Biotechnology & nanotechnology
Akbar Safipour Afshar; fateme Saied Nematpour
Volume 27, Issue 4 , November and December 2020, , Pages 550-558
Abstract
Introduction: Green synthesis of silver nanoparticles is a simple and low-cost method. In addition, finding new therapeutic effects for silver nanoparticles is considered as an attractive field in cancer research. In the present study, synthesis of silver nanoparticles was performed by aqueous leaf extract ...
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Introduction: Green synthesis of silver nanoparticles is a simple and low-cost method. In addition, finding new therapeutic effects for silver nanoparticles is considered as an attractive field in cancer research. In the present study, synthesis of silver nanoparticles was performed by aqueous leaf extract of barberry. The effects of Ag nanoparticles were evaluated on cell viability and expression levels of ODC1 gene of the human breast cancer cell line (MDA-MB-231).
Materials and Methods: The synthetic nanoparticles were analyzed by UV-vis spectrophotometry and Transmission Electron Microscopy. The cultured cancer cells were treated under different concentrations of synthesized nanoparticles.The viability of the cells was analyzed by MTT assay and relative expression of ODC1 gene by Real-Time PCR.
Results: TEM photomicrographs showed that the spherical silver nanoparticles have been synthesized with an average size of 16 nm. The results showed cytotoxicity of silver nanoparticles in a time and dose-dependent manner. The average IC50 calculated was 16.48 µg/ml. The results also showed that silver nanoparticles decreased ODC1 gene expression levels about 4 folds at 20 µg/ml nanoparticles compared to control.
Conclusion: It seems that green synthesized silver nanoparticles through the gradual release of silver ions in the acidic environment of the cancer cells and production of oxygen radicals, as well as reduction of ODC1gene expression cause diminish of cancer cell viability.
Biotechnology & nanotechnology
Elham Sasani; Hashem Shahi Malmir; Fatemeh Daneshmand; Mohammad Majdizadeh; Bibi Fatemeh Haghiralsadat
Volume 27, Issue 1 , May and June 2020, , Pages 35-47
Abstract
Introduction: Lipid nano-carriers with slow release and semi-targeted delivery of the drug can reduce some of the challenges of drug delivery to cancer cells. The aim of this study was to prepare and evaluate physiochemicals of various formulations of the liponiosomal system containing doxorubicin in ...
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Introduction: Lipid nano-carriers with slow release and semi-targeted delivery of the drug can reduce some of the challenges of drug delivery to cancer cells. The aim of this study was to prepare and evaluate physiochemicals of various formulations of the liponiosomal system containing doxorubicin in order to achieve targeted formulation in order to better fight cancer cells.Methods: Nano-carriers were synthesized using different molar ratios of structural elements such as Dipalmitoylphosphatidylcholine, Cholesterol, Sorbitan ester and 3 out of 5 synthesized formulation were chose based on encapsulation efficiency. Then, in order to determine the final formula, release profiles comprised by dialysis and spectroscopy methods. The final formula was PEGylated by using DSPE-mPEG(2000) in order to investigate its effects on encapsulation efficiency and release profiles in healthy and cancerous cells simulated environment. Physiochemical characteristics such as size, zeta potential, Polydispersity Index(PDI), IR spectrum, and morphology were identified.Results: The final PEGylated formula encapsulating doxorubicin had 102.9 nm size, 94.17±3.21% encapsulation efficiency, -6.67 mV zeta potential and 0.128 PDI. The maximum release rate of the drug for this nano-carrier in healthy and cancerous within 48 hours was 24.43% and 26.81% respectively. IR and morphological investigations showed no chemical interaction between drug and nanocarrier and the particles are spherical in shape. Conclusion: The result of this research indicates that the PEGylated liponiosomal system, having the appropriate physiochemical properties, has not changed the chemical nature of drug and thus can be a suitable and semi-targeted carrier for doxorubicin.
Biotechnology & nanotechnology
Amirhossein Barghi; Hossein Honari; Mohamadali Ebrahimi; Gholamreza Bakhshi Khaniki; Seyed Mojtaba aghaee
Volume 27, Issue 1 , May and June 2020, , Pages 103-112
Abstract
Introduction: Saponaria officinalis have various saponin isoforms. Saponin is a ribosome-inactivating protein (RIP). The SO9 isoform of saponins depurinates the adenine 4324 in the preserved GAGA sequence resulting in impairment of protein production. In this study, the S09 isoform was expressed in E. ...
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Introduction: Saponaria officinalis have various saponin isoforms. Saponin is a ribosome-inactivating protein (RIP). The SO9 isoform of saponins depurinates the adenine 4324 in the preserved GAGA sequence resulting in impairment of protein production. In this study, the S09 isoform was expressed in E. coli and its antibody titers were evaluated in Mouse. Methods: The S09 gene was synthesized and isolated from the pUC57-S09 recombinant plasmid using the restriction enzymes BamH1 and Sal1, and then cloned in the expression vector pET28a(+). Expression of the new recombinant protein was induced by IPTG. The recombinant S09 protein was purified by Ni affinity chromatography. The recombinant protein was confirmed through western blotting. The Mouse were vaccinated through intraperitoneal injection of the purified protein and serum IgG titer was measured through ELISA.Results: Subcloning of S09 gene in the pET28a(+) expression vector was confirmed by PCR and enzymatic digestion. The presence of 29 kDa protein band in SDS-PAGE showed the high expression of recombinant protein. The recombinant S09 protein was detected by polyclonal antibody. After injection of the protein to the test groups, the antibody titer was measured by ELISA. Conclusion: The adjuvant property and immunogenicity of the purified recombinant S09 antigen showed that this antibody can be used to detect the presence of S09 in Saponaria officinal is, as a candidate for vaccine, for production of diagnostic kits, and in human cells anticancer studies.
Biotechnology & nanotechnology
Madjid Momeni-Moghaddam; Roya Gholami
Volume 26, Issue 5 , November and December 2019, , Pages 651-656
Abstract
Abstract:Aims: Collagen synthesis can help to heal wounds, especially Large wounds. Any treatments that can stimulate collagen synthesis in fibroblasts can accelerate wound repair, so it has clinical value. Stem cells secrete some macromolecules and signals that can have different effects in other cells.Materials ...
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Abstract:Aims: Collagen synthesis can help to heal wounds, especially Large wounds. Any treatments that can stimulate collagen synthesis in fibroblasts can accelerate wound repair, so it has clinical value. Stem cells secrete some macromolecules and signals that can have different effects in other cells.Materials and Methods: In this study, human mesenchymal stem cells were cultured and their medium supernatant was harvested as a medium containing the signal. After determining concentration, they were exposed to mouse fibroblasts. The cell viability was examined and then in the molecular section, by designing specific primers, three The collagen gene was evaluated by Real-Time PCR.Results: The results indicated a growth-promoting effect using the amount of 20 μg /ml of stem cells conditioned media in the culture medium of fibroblast cells. It was also found in the molecular section, that this concentration could increase the expression of all three selected collagen genes.Discussion and conclusion: The results of this study fully confirmed the initial goals, indicating the high power of mesenchymal stem cells in the induction of collagen synthesis, and thus could be a very good candidate for clinical studies.
Biotechnology & nanotechnology
Marzieh Varasteh Shams; Farhad Nazarian-Firouzabadi; Ahmad Ismaili
Volume 26, Issue 4 , November and December 2019, , Pages 404-412
Abstract
New and effective antimicrobial agents are inevitable because of the increased use of antibiotics and spread of microbial resistance. The expression of antimicrobial peptides that are part of the inherent immune system of all organisms, is a novel approach of confronting a wide range of pathogens. Among ...
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New and effective antimicrobial agents are inevitable because of the increased use of antibiotics and spread of microbial resistance. The expression of antimicrobial peptides that are part of the inherent immune system of all organisms, is a novel approach of confronting a wide range of pathogens. Among the wide range of antimicrobial peptides, Dermaseptin B1 is a potent cationic peptide with strong antimicrobial activity. In this study, Dermaseptin B1 (DrsB1) coding sequence from Phyllomedusa bicolor frogs was fused to a tandem repeat of a chitin-binding domain (CBD) from Cladosporium fulvum Avr4 effector protein and expressed in tobacco Hairy Roots (HRs). The expression of recombinant (CBD)2-DrsB1 peptide was confirmed, using molecular methods and its antimicrobial activity was evaluated against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis and Bacillus subtilis bacteria. The results of antimicrobial activity analysis demonstrated that the recombinant protein had a significant (P
Biotechnology & nanotechnology
Zeinab Noori; Akbar Safipour Afshar
Volume 26, Issue 4 , November and December 2019, , Pages 527-534
Abstract
Aim: The characteristics of cancer cells that differentiate them from normal cells can be attributed to the increase of the ornithine decarboxylase enzyme (ODC), which has a very important role in the metabolism of polyamines in cancer cells. In this research, we tried to study the effects of cytotoxicity ...
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Aim: The characteristics of cancer cells that differentiate them from normal cells can be attributed to the increase of the ornithine decarboxylase enzyme (ODC), which has a very important role in the metabolism of polyamines in cancer cells. In this research, we tried to study the effects of cytotoxicity of Rhubarb extract on three different solvents distilled water, ethanol and n-hexane on MCF-7 cell lines. Also, the rate of expression of the ODC1 gene as one of the genes involved in breast cancer was studied under the influence of the extract of this plant.Materials and Methods: MCF-7 breast cancer cells were cultured in DMEM medium. Subsequently, they were subjected to increasing concentrations of extract from 0-200 μg/mL. The cytotoxic activity of the extract was investigated using MTT assay. The quantitative analysis of the ODC1 gene was performed using the Real Time-PCR.Results: Cell line mortality increases significantly in a concentration-dependent manner. In the rhubarb extracts, the most cytotoxicity was observed in 72 hours. Changes in ODC1 gene expression in MCF-7 cell lines treated with different concentrations of rhubarb extract indicate a decrease in gene expression with increasing concentrations of extracts.Conclusion: The results of this study indicate that Rhubarb extract has a potential influence on ODC1 gene expression in breast cancer cells and cause inhibition of cancer cell proliferation.