نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی ارشد، گروه مهندسی شیمی و نفت، دانشگاه آزاد اسلامی، واحد قوچان، قوچان، ایران.
2 استادیار، گروه مهندسی شیمی و نفت، واحد قوچان، دانشگاه آزاد اسلامی، قوچان، ایران.
چکیده
اهداف در سال‌های اخیر، یافتن ترکیبات جدید با منشأ گیاهی در درمان و پیشگیری از بیماری‌ها، مدنظر محققان قرار گرفته است. هدف مطالعه حاضر بررسی توان آنتی‌اکسیدانی و ضدالتهابی گیاهان ناخنک و زبان گنجشک است که در طب سنتی استفاده می‌‌شود.
مواد و روش ها در این پژوهش، پس از تهیه عصاره اتانولی گیاهان ناخنک و زبان گنجشک به روش خیساندن، میزان توان آنتی‌اکسیدانی گیاهان با روش ارزیابی احیای یون مس و قدرت ضدالتهابی آن‌ها با آزمون ممانعت از تغییر شکل پروتئین آلبومین گاوی، اندازه‌گیری شد.
یافته ها نتایج نشان داد هر دو گیاه اثر آنتی‌اکسیدانی و ضدالتهابی دارند. فعالیت آنتی‌اکسیدانی و ضدالتهابی به غلظت عصاره‌ها وابسته است و با افزایش غلظت عصاره‌ها، خاصیت آنتی‌اکسیدانی و ضدالتهابی آن‌ها افزایش می‌یابد. بیشترین میزان ترکیبات فنلی در هر گرم عصاره خشک گیاه زبان گنجشک 047/0 میلی‌گرم است. این گیاه بیشترین اثر آنتی‌اکسیدانی (045/0±714/0) را دارد. بیشترین میزان ترکیبات فلاونوئیدی در گیاه ناخنک 23/9 میلی‌گرم است. این گیاه بیشترین اثر ضدالتهابی ( 033/0±217/0) را دارد.
نتیجه گیری استفاده از روش‌های غربالگری همانند روش‌های ذکرشده، فرصتی برای یافتن ترکیبات فعال با چند اثر متفاوت را فراهم خواهد کرد.
کلیدواژه‌ها
عنوان مقاله [English]
Evaluation of Phenolic Compounds and Assessment of Antioxidant Capacity and Anti-Inflammatory of Alcoholic Extraction of Melilotus Officinalis and Fraxinus Excelsior
نویسندگان [English]
- Fariba Zarei 1
- Susan Khosroyar 2
1 MSc. Student, Department of Chemical and Petroleum Engineering, Islamic Azad university, Quchan Branch, Quchan, Iran.
2 Assistant Professor, Department of Chemical and Petroleum Engineering, Quchan Branch, Islamic Azad University, Quchan, Iran.
چکیده [English]
Objectives In recent years, researchers tried to find new compounds with herbal origins to treat and prevent diseases. The current study aimed at investigating the antioxidant and anti-inflammatory effects of Melilotus officinalis and Fraxinus excelsior alcoholic extracts, commonly used in traditional medicines.
Methods After preparing the ethanolic extracts of M. officinalis and F. excelsior by the maceration method, the antioxidant properties were investigated by the cupric ion reducing assay (cupric assay) and the anti-inflammatory effects were also investigated through the prevention test of bovine serum albumin (BSA) and its transformation potential.
Results The obtained results showed that both mentioned plants had antioxidant and anti-inflammatory effects. The antioxidant properties changed based on the concentration of extracts, and antioxidant and anti-inflammatory properties increased along with the concentration increase. The highest amount of phenolic compound in F. excelsior was 0.04 mg/g of dry extract; this plant also showed the highest antioxidant effect (0.714±0.045). The highest amount of flavonoid compounds in M. officinalis was 9.23 mg; this plant showed the highest anti-inflammatory effects (0.21±0.033 ).
Conclusion The employed screening methods were useful to find biological components with different properties.
کلیدواژه‌ها [English]
- Antioxidant
- Anti-inflammatory
- Melilotus officinalis
- Fraxinus excelsior
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