نوع مقاله : مقاله پژوهشی
نویسندگان
1 دکترای حرفه‌ای، باشگاه پژوهشگران جوان و نخبگان، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران.
2 استادیار، گروه شیمی، دانشکده علوم، دانشگاه زابل، زابل، ایران.
3 استادیار، گروه علوم بالینی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران.
4 دانشجوی دکترای حرفه ای، گروه فارماکولوژی، دانشکده داروسازی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.
چکیده
اهداف مقاومت دارویی در باکتری‌های پاتوژن مشترک بین انسان و دام خطری است که رونق و سودآوری صنعت دام‌پروری و سلامت جامعه را تهدید می‌کند. برای مقابله با این تهدید، شناسایی و کاربرد ترکیبات ضدباکتریایی جدید روی باکتری‌های مقاوم ضروری است. مشتقات تیازول و ایمیدازول ترکیبات ضدباکتریایی جدیدی هستند. در این مطالعه اثر ضدباکتریایی یک مشتق تیازول و دو مشتق ایمیدازول بر باکتری‌های رودوکوکوس اکوئی و بروسلا آبروتوس و پاستورلا مولتی‌سیدا بررسی شده است.
مواد و روش‌ها برای بررسی اثر ضدباکتریایی مشتقات، از روش انتشار در دیسک برای اندازه‌گیری قطر هاله مهار رشد و از روش رقت‌سازی در پلیت 96 خانه برای تعیین حداقل غلظت مهارکننده رشد استفاده شد.
یافته‌ها نتایج تحقیق نشان داد هیچ‌یک از مشتقات تیازول و ایمیدازول بر باکتری بروسلا آبروتوس اثر مهاری نداشتند. مشتقات ایمیدازول نیز هیچ‌گونه اثر مهاری بر باکتری‌ها نداشتند. اثر مهاری تنها از مشتق تیازول با 128 و 16 میکروگرم بر میلی‌لیتر و قطر هاله مهار رشد2/3±0/19 و 3/0±1/28 میلی متر به ترتیب بر باکتری‌های رودوکوکوس اکوئی و پاستورلا مولتی‌سیدا مشاهده شد.
نتیجه‌گیری اثر مهاری مشتق تیازول بر دو باکتری پاستورلا مولتی‌سیدا و رودوکوکوس اکوئی مشاهده شد که این اثر مهاری بر باکتری پاستورلا مولتی‌سیدا بسیار قوی‌تر بود.
کلیدواژه‌ها
عنوان مقاله [English]
Study on Antibacterial Effect of New Thiazole and Imidazole Derivatives on Zoonoses Bacterial Pathogen
نویسندگان [English]
- Behzad Ghasemi 1
- Hamid Beyzaei 2
- Seyed Hadi Hashemi 3
- Vahid Reza Askari 4
1 DVM, Young Researchers and Elite Club, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.
2 Assistant Professor, Department of Chemistry, Faculty of Sciences, University of Zabol, Zabol, Iran.
3 Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran.
4 DP Candidate, Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
چکیده [English]
Backgrounds Drug resistance in zoonotic bacterial pathogens is a serious risk, which threatens the prosperity and profitability of livestock industry and also public health. To confront this threat, it is necessary to determine and use novel antibacterial compounds on the resistant bacteria. Thiazole and imidazole derivatives are the novel antibacterial agents; the current study aimed at assessing the antibacterial effect of 1 thiazole derivative and 2 imidazole derivatives on Rhodococcus equi, Brucella abortus, and Pasteurella multocida.
Methods & Materials To investigate the antibacterial effect of selected antibiotics, the disk diffusion method was used and the diameter of growth inhibition zone was also measured; the serial dilution method in micro-plates was also employed to assess the minimum inhibitory concentrations (MICs).
Results The results showed that none of the thiazole and imidazole derivatives had inhibitory effect on B. aabortus. Imidazole derivatives also lacked any inhibitory clue on the tested bacteria. The inhibitory effect was only observed in the thiazole derivative with MICs of 128 and 16 µg/mL, and growth inhibition zone diameter of 19.3±0.2 and 28.1±0.3 mm on R. equi and P. multocida, respectively.
Conclusion The inhibitory effect of thiazole derivative was observed on R. equi and P. multocidawas. This antibiotic also showed more inhibitory effects on P. multocida.
کلیدواژه‌ها [English]
- Antibacterial
- Thiazole
- Imidazole
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