نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد، گروه زیست‌شناسی سلولی و مولکولی، دانشکده زیست‌شناسی، دانشگاه دامغان، دامغان، ایران.
2 استادیار، گروه زیست‌شناسی سلولی و مولکولی، دانشکده زیست‌شناسی، دانشگاه دامغان، دامغان، ایران.
3 استادیار، گروه زیست‌شناسی عمومی، دانشکده زیست‌شناسی، دانشگاه دامغان، دامغان، ایران.
چکیده
اهداف استرس اکسیداتیو در کشت آزمایشگاهی اجتناب‌ناپذیر است. بنابراین هدف از مطالعه حاضر بررسی اثر یوبیکوینون به‌عنوان آنتی‌اکسیدانتی قوی بر پراکسیداسیون لیپیدی و نرخ تکوین فولیکول‌های پره‌آنترال موش سوری در شرایط کشت آزمایشگاهی است.
مواد و روش ها در این پژوهش فولیکول‌های پره‌آنترال از تخمدان موش‌های 14 تا 16 روزه به روش مکانیکی جدا (123n=) و به دو گروه کنترل (78=n) و تیمارشده با یوبیکوینون (45n=) تقسیم شدند. فولیکول‌های پره‌آنترال با حضور یا عدم حضور (کنترل) 50 میکرومولار یوبیکوینون کشت و تخمک‌گذاری در روز دوازدهم کشت القا شد، سپس نرخ رشد، بقا، تشکیل حفره آنتروم، تخمک‌گذاری و تخمک MII ارزیابی شد. به‌طور جداگانه مالون دی‌آلدئید (MDA) به‌عنوان نشانگر زیستی پراکسیداسیون لیپیدی در شروع کشت، در ساعت‌های 24، 48، 72 و 96 بررسی شد. داده‌ها با استفاده از نرم‌افزار SPSS و آزمون t مستقل تجزیه‌وتحلیل شد.
یافته ها در این پژوهش نرخ بقا و رشد فولیکول‌های پره‌آنترال تیمارشده با یوبیکوینون به‌طور معناداری بیشتر از گروه کنترل بود. نرخ تشکیل حفره آنتروم و تخمک‌گذاری و تخمک‌های MII در حضور یوبیکوینون به‌طور معناداری نسبت به گروه کنترل بیشتر بود، درحالی‌که مقدار مالون دی‌آلدئید فولیکول‌های تیمارشده با یوبیکوینون در مقایسه با گروه کنترل به‌طور معناداری کمتر بود.
نتیجه گیری محیط کشت غنی‌شده با یوبیکوینون، تکوین فولیکول‌های پره‌آنترال را با کاهش پراکسیداسیون لیپیدی بهبود می‌دهد.
کلیدواژه‌ها
عنوان مقاله [English]
The Effect of Ubiquinone on Malondialdehyde Content of Mouse Preantral Follicles During in Vitro Culture
نویسندگان [English]
- Roya Hedayati Kashka 1
- Saeed Zavareh 2
- Taghi Lashkabolouki 3
1 MSc. Student, Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran.
2 Assistant Professor, Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran.
3 Assistant Professor, Department of General Biology, School of Biology, Damghan University, Damghan, Iran.
چکیده [English]
Background Oxidative stress is unavoidable during in vitro culture. The present study aimed to investigate the effect of ubiquinone as a potent antioxidant on lipid peroxidation and development rate of mice in vitro cultured preantral follicles.
Materials & Methods Preantral follicles were isolated mechanically from 14- to 16-day-old mice (n=123) and divided into control (n=78) and ubiquinone treated groups (n=45). Preantral follicles were cultured in the presence or absence (control) of 50 µM ubiquinone. Ovulation was induced at 12th day. The rates of growth, survival, antrum formation, ovulation, and MII oocytes were evaluated. Separately, malondialdehyde (MDA) as a biomarker of lipid peroxidation was assessed at different time points of 24, 48, 72, and 96 h. Statistical analysis was performed by Independent t test through using SPSS.
Results The growth and survival rate of ubiquinone treated preantral follicles was significantly higher than those of the control group. The rates of antrum formation, ovulation, and MII oocytes in the presence of ubiquinone were significantly higher than those of the control group. Whereas, MDA levels of ubiquinone treated preantral follicles was significantly lower compared with that of the control group.
Conclusion Supplemented culture medium with ubiquinone improves the development of preantral follicles by reducing the lipid peroxidation.
کلیدواژه‌ها [English]
- Ubiquinone
- Preantral follicles
- Lipid peroxidation
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