نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار گروه علوم آزمایشگاهی ، دانشگاه آزاد اسلامی واحد چالوس، چالوس ،ایران
2 کارشناس ارشد میکروب شناسی، دانشگاه آزاد اسلامی واحد چالوس، چالوس ،ایران
3 دانشیار گروه میکروب شناسی، دانشگاه آزاد اسلامی واحد تنکابن،نکابن ،ایران
4 دانشیار گروه داخلی ، دانشگاه علوم پزشکی بابل ،بابل، ایران
چکیده
زمینه و هدف: استرپتوکوکوس گالولیتیکوس یکی از باکتری‌های مرتبط با ایجاد سرطان کولورکتال در انسان می‌باشد. هیچ مطالعه‌ای برای شناسایی این باکتری در روده بزرگ با استفاده از آزمون PCR و مقایسه آن با روش کشت میکروبی در افراد تحت کولونوسکوپی در شمال ایران انجام نشده است.
مواد و روش‌ها: در یک مطالعه توصیفی برای تشخیص Streptococcus gallolyticus از 55 فرد تحت کولونوسکوپی مراجعه‌کننده به بیمارستان‌های بابل و چالوس نمونه بیوپسی اخذ گردید. به‌منظور تشخیص باکتری پس از استخراج DNA ابتدا از پرایمرهای طراحی‌شده (PCO3, PCO4) به‌منظور تحلیل کیفی DNA استخراج‌شده استفاده شد و سپس ژن اختصاصی Streptococcus gallolyticus تکثیر یافت. علاوه بر کشت از آزمون‌های تشخیصی، مثل رنگ‌آمیزی گرم، آزمون کاتالاز، هیدرولاز، هیدرولیز هیپورات و هیدرولیز اسکولین استفاده شد.
یافته‌ها: در این مطالعه توصیفی از 55 نمونه بیوپسی افراد تحت کولونوسکوپی، 3 نمونه (5/5 درصد) با فاصله اطمینان 95 درصد مثبت و 52 مورد (94/5 درصد) از نظر وجود DNA Streptococcus gallolyticus منفی گزارش شدند. رابطه معنادار بین دو روش تشخیصی کشت و PCR مشاهده گردید (015/ 0 p.value:). در بررسی‌های انجام‌شده در روش کشت، رابطه معنی‌داری بین سابقه کلورکتال کنسر فامیلی، دیابت و وجود باکتری Streptococcus gallolyticus وجود داشت.
نتیجه‌گیری: کاربرد همزمان دو روش، در مواردی که حصول نتیجه سریع مدنظر است، توصیه می‌شود.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Detection of Streptococcus Gallolithicus in People Undergoing Colonoscopy by Chain Reaction (PCR) and Culture
نویسندگان [English]
- haniyeh bashi fakhar 1
- Foroogh Eshaghi kojor 2
- Masoud Ghane 3
- javad Shokri 4
1 Assistant Professor, Department of Laboratory Sciences, Islamic Azad University, Chalous Branch, Chalous, Iran
2 Master of Microbiology, Islamic Azad University, Chalous Branch, Chalous, Iran
3 Associate Professor, Department of Microbiology, Islamic Azad University, Tonekabon Branch, Nekabon, Iran
4 Associate Professor, Department of Internal Medicine, Babol University of Medical Sciences, Babol, Iran
چکیده [English]
Introduction: Streptococcus galloliticus is one of the bacteria associated with colorectal cancer in humans. No studies have been performed to identify this bacterium in the large intestine using PCR test and compare it with microbial culture in patients undergoing colonoscopy in northern Iran.
Materials and Methods: In a descriptive study to diagnose Streptococcus gallolyticus, biopsy specimens were obtained from 55 individuals undergoing colonoscopy referred to Babol and Chalous hospitals. To detect bacteria after DNA extraction, first designed primers (PCO3, PCO4) were used to qualitatively analyze the extracted DNA and then the specific gene of Streptococcus gallolyticus was amplified. In addition to culture, diagnostic tests such as gram staining, catalase test, hydrolase, hydrate hydrolysis and scolin hydrolysis were used.
Results: In this descriptive study, out of 55 biopsy specimens of individuals undergoing colonoscopy, 3 specimens (5.5%) with 95% confidence interval were positive and 52 cases (5.94%) were negative for Streptococcus gallolyticus DNA. There was a significant relationship between the two diagnostic methods of culture and PCR (p.value 0.015).
Conclusion: The simultaneous application of the two methods is recommended in cases where the result is rapid.
کلیدواژه‌ها [English]
- Streptococcus gallolithicus
- Colonoscopy
- Molecular
- Culture
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