نوع مقاله : مقاله پژوهشی
نویسندگان
- رمضان صادقی 1
- مهربان صادقی 2
- رحمان عبدی‌زاده 3
- مرتضی سدهی 4
- مرتضی هاشم زاده 5
- شهربانو پرچمی 6
- محمد رسول اسدی امیرآبادی 7
1 استادیار گروه مهندسی بهداشت محیط، دانشکده بهداشت، دانشگاه علوم پزشکی شهرکرد. ایران
2 استاد گروه مهندسی بهداشت محیط، دانشکده بهداشت، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران
3 دانشیار گروه انگلشناسی و قارچشناسی، دانشکده پزشکی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران
4 دانشیار گروه اپیدمیولوژی و آمار زیستی، دانشکده بهداشت، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران
5 استاد ژنتیک، گروه ژنتیک و بیولوژی مولکولی، دانشکده پزشکی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران
6 کارشناس ارشد بیوتکنولوژی، مرکز تحقیقات سلولی و مولکولی، دانشکده پزشکی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران
7 کارشناس ارشد مهندسی بهداشت محیط، دانشکده بهداشت، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران
چکیده
زمینه و هدف: فلزات سنگین به دلیل ماهیت سمی همانند برخی مواد آلی در محیط پاکسازی نمی‌شوند و دسته‌ای از آلاینده‌های پایدار و غیرقابل تجزیه بیولوژیکی را تشکیل می‌دهند. این عناصر در نتیجه فعالیت‌های طبیعی مانند فوران آتشفشان‌ها و فعالیت‌های انسانی مثل استخراج معادن، تولید پساب‌های صنعتی، فاضلاب‌های شهری و برخی از آفت کش‌ها وارد محیط شده و می‌توانند به زنجیره غذایی راه پیدا کنند.
مواد و روش ها: در این مطالعه باکتری‌های مقاوم به کروم (VI) از فاضلاب صنعتی مجتمع فولاد مبارکه اصفهان جداسازی شدند. سویه‌های جداسازی شده، شناسایی و میزان مقاومت آنها نسبت به کروم تعیین و از سویه‌ای که بالاترین مقاومت را به کروم نشان داد به منظور حذف زیستی کروم استفاده شد. روش تجزیه و تحلیل داده‌ها با استفاده از آمار توصیفی و واریانس یک‌طرفه یا ANOVA می‌باشد.
یافته ها: نتایج این مطالعه منجر به شناسایی پنج سویه مقاوم به فلز کروم شد، که باکتری Micrococcus luteus SEHD031RS به دلیل حداقل غلظت بازدارندگی 140 میلی گرم بر لیتر و حداقل غلظت کشندگی 152 میلی گرم بر لیتر، برترین سویه مقاوم به کروم شناخته شد. در این مطالعه بالاترین میزان حذف فلز کروم در 4=pH، غلظت 30 میلی گرم بر لیتر و مدت زمان 96 ساعت، 5/82 درصد حاصل شد.
نتیجه گیری: نتایج حاصل از این مطالعه نشان می‌دهد سویه‌ی Micrococcus luteus SEHD031RS می‌تواند به عنوان یک میکروارگانیسم موثر در حذف کروم از فاضلاب‌های صنعتی و یا پاکسازی محیط استفاده شود.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Investigation of Biological Removal of Chromium (VI) Using the Isolated Bacteria from Industrial Wastewater of Mobarakeh Steel Complex in Isfahan
نویسندگان [English]
- Ramezan Sadeghi 1
- Mehraban Sadeghi 2
- Rahman Abdizadeh 3
- Morteza Sedehi 4
- Morteza Hashemzadeh Chaleshtori 5
- Shahrbanou Parchami Barjui 6
- Mohammad Rasoul Asadi Amirabadi 7
1 PhD in Environmental Toxicology . Environmental Health Engineering Dept., Shahrekord University of Medical Sciences, Shahrekord, I.R. Iran.
2 PhD in Environmental Health Engineering. Environmental Health Engineering Dept., Shahrekord University of Medical Sciences, Shahrekord, I.R. Iran
3 Associate Professor, Parasitology Dept., Shahrekord University of Medical Sciences, Shahrekord, Iran
4 Associate Professor, Epidemiology and Biostatistics Dept., Shahrekord University of Medical Sciences, Shahrekord, Iran
5 Professor of Genetics Department of Genetics and Molecular Biology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
6 Master of Biotechnology, Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
7 Master of Environmental Health Engineering, Shahrekord University of Medical Sciences, Shahrekord, Iran
چکیده [English]
Backgrounds: Similar to some organic materials the heavy metals are not easily removed from the environment because of the toxic nature and form a class of stable and non-biodegradable pollutants. These elements enter the nature and the food chain as a result of natural activities such as eruptions of volcanoes and human activities such as mining, producing industrial and urban wastewaters and applying pesticides.
Materials and Methods: In this study, chrome (VI) -resistant bacteria were isolated from the industrial wastewater taken from Isfahan Mobarakeh Steel Complex. The isolated strains were identified and their resistance to chromium was determined and the strain with the highest resistance to chromium was used for its bioremediation.Data were analyzed using descriptive statistics and one way ANOVA.
Results: The results of this study led to the identification of five chrome resistant strains that the Micrococcus luteus SEHD031RS bacterium was detected as the best chrome resistant strain due to the minimum inhibitory concentration of 140 mgL-1 and minimum bactericidal concentration of 152 mgL-1. In this study, the highest chromium removal rate of 82.5% was obtained at pH 4, concentration of 30 mgL-1 and 96 hours.
Conclusion: The results of this study indicate that the Micrococcus luteus SEHD031RS strain can be used as an effective microorganism in removing chromium from industrial wastewater or environmental bioremediation.
کلیدواژه‌ها [English]
- Industrial wastewater
- Heavy metals
- Chromium (VI)
- Biological removal
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