تأثیر یک دوره تمرین تناوبی شدید بر مقادیر سرمی TNF-α و بیان ژن FOXO1 هیپوکمپ موش‌های صحرایی دیابتی نر نژاد ویستار

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران

2 استادیار فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران

چکیده

زمینه و هدف: افزایش بیان ژن FOXO1 در هیپوکمپ و همچنین عامل التهابی TNF-α در افراد دیابتی باعث بیان ژن‌های درگیر در آپوپتوزشده و سبب تخریب عملکرد اجرایی آن می‌شود. هدف از پژوهش حاضر، بررسی تأثیر 6 هفته تمرین تناوبی شدید بر بیان ژن FOXO1 در هیپوکمپ و میزان سرمی TNF-α موش‌های صحرایی دیابتی نر ویستار بود.
مواد و روش‌ها: برای این مطالعه، تعداد 48 سر موش صحرایی نر ویستار (4 هفته‌ای) با وزن10±150 گرم در 4 گروه 12 تایی: موش‌های دیابتی، دیابت تمرین، کنترل و کنترل تمرین دسته‌بندی شدند. به‌منظور القای دیابت از روش تزریق صفاقی محلول STZ (50 mg/kg) استفاده گردید. پس از یک هفته آشناسازی با محیط و تمرین، پروتکل تمرین تناوبی شدید شامل 3 روز تمرین در هفته به مدت 6 هفته با شدت 50 تا 110 درصد حداکثر اکسیژن مصرفی انجام شد. 24 ساعت بعد از اتمام تمرین، آزمون عملکردی گرفته شد و 48 ساعت پس از آزمون عملکردی، حیوانات تشریح شدند. بیان ژن FOXO1 با استفاده از تکنیک Real Time PCR ارزیابی گردید.
یافته‌ها: با توجه به نتایج به‌دست‌آمده تمرین تناوبی شدید باعث کاهش معنی‌دار بیان ژن FOXO1 در گروه‌های تمرین شد (P≤0/01 ). همچنین پروتئین TNF-α به‌طور معنی‌داری کاهش یافت (P≤0/05). افزایش وزن هیپوکمپ همراه با کاهش ژن FOXO1 در گروه دیابت تمرین تناوبی شدید مشاهده شد.
نتیجه‌گیری: براساس یافته‌های پژوهش تمرین تناوبی شدید احتمالاً با کاهش عوامل آپوپتوزیFOXO1 و التهابی TNF-α از کاهش وزن بافت هیپوکمپ که بر اثر دیابت اتفاق می‌افتد، جلوگیری می‌کند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of High Intensity Interval Training on Serum TNF-α Levels and FOXO1 Gene Expression of Hippocampus in Male Diabetic Wistar Rats

نویسندگان [English]

  • soudabeh rezaei 1
  • Neda Khaledi 2
1 Master Student of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
2 Associate professor in exercise Physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
چکیده [English]

Introduction: Increasing the expression of FOXO1 gen in hippocampus as well as inflammatory factor of TNF-α in diabetic individual's causes the expression of genes involved in apoptosis and disrupts its performance. The aim of the present study was investigation of the effect of 6-weeks HIIT on FOXO1 gene expression in the hippocampus and level of serum TNF-α in male Wistar diabetic rats.
Materials and Methods: For this study, 48 male Wistar rats (4 weeks old) with 150±10 g weight were categorized in 4 groups of (n=12): diabetic rats, exercise diabetic, control and exercise control. For induction of diabetes, peritoneal injection of STZ solution (50 mg/kg) was used. After a week of familiarization with the environment and practice, HIIT protocol consisted of 3 days per week for 6 weeks with 50% to 110% of maximum oxygen consumption was performed. 24 hours after the completion of the exercise, the functional test was taken and the animals were autopsy 48 hours after the functional test. FOXO1 gene expression was evaluated using Real Time PCR technique.
Results: Regarding the results, HIIT significantly decreased FOXO1 gene expression in training groups (P≤0.01). Also, TNF-α protein decreased significantly (P≤0/05). Weight gain of the hippocampus was associated with a reduction in the FOXO1 gene in the diabetic training group.
Conclusion: HIIT probably reduce the weight loss of the hippocampal tissue that caused by diabetes, by reducing the FOXO1 apoptotic and inflammatory factors of TNF-α.

کلیدواژه‌ها [English]

  • High intensity interval training
  • TNF-α
  • apoptosis
  • Time to exhaustion
  • neuronal loss
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