بررسی استریولوژیک حجم ساختارهای کلیه در پانکراتیت القا شده توسط ال- آرژینین و نقش درمانی پانکراتین

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار علوم تشریحی دامپزشکی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران

2 دانشجوی دکترای دامپزشکی، دانشکده‌ دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران

چکیده

زمینه و هدف  هدف از پژوهش حاضر، ارزیابی استریولوژیک تأثیر حفاظتی پانکراتین بر تغییرات مورفولوژیک ایجاد شده در ساختارهای کلیوی موش به‌دنبال تیمار با ال- آرژینین بود.
مواد و روش‌هابرای انجام تحقیق، ۵۰ موش سوری نر بالغ با وزن 2±30 گرم به ۵ گروه (مساوی) دسته‌بندی شدند. در گروه کنترل، هیچ‌گونه تجویزی صورت نگرفت. گروه‌های القای پانکراتیت، 2 دوز 300 و 400 میلی‌گرم در 100 گرم وزن بدن ال- آرژینین دریافت کردند. به گروه‌های القای پانکراتیت با 2 دوز متفاوت، داروی درمانی 5/3 واحد لیپاز- پانکراتین تجویز گردید. تمام تیمارها به‌مدت 3 روز متوالی یک‌بار در روز، محلول ال- آرژینین دریافت کرده و 72 ساعت بعد، محلول لیپاز- پانکراتین به مدت 20 روز به گروه‌های پانکراتیت تجویز گردید.
یافته‌ها نتایج نشان داد که به‌دنبال پانکراتیت تجربی، به‌استثنای حجم بخش مرکزی، کاهش حجم قشر کلیه، حجم لوله‌های پیچیده‌ نزدیک و دور و نیز گلومرول‌ها در گروه‌های تیمار نسبت به گروه کنترل دیده شد. با تجویز لیپاز- پانکراتین همراه با ال-آرژینین، مقادیر میانگین حجم فاکتورهای بخش قشری، لوله‌های پیچیده‌ نزدیک و گلومرول‌ها به گروه کنترل نزدیک شده بود.
نتیجه‌گیری براساس بررسی نهایی یافته‌ها، محلول لیپاز- پانکراتین می‌تواند تا حدی در کاهش تأثیرات پانکراتیت القا شده توسط دوز بالای ال- آرژینین مؤثر باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Stereological Study of the renal structures volume in L-arginine-induced pancreatitis and therapeutically effect of pancreatin

نویسندگان [English]

  • Rahmat allah Fatahian dehkordi 1
  • Mohammad Javad Yazdanpanah 2
  • Reza Khetvan 2
1 Associate professor, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
2 Student of veterinary medicine, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
چکیده [English]

Introduction: The aim of this study was to stereological evaluate of the protective effect of pancreatin on morphological changes in mice kidney after treatment with L-arginine .
Materials and Methods: To conduct research, 50 adult male rats weighing 30±2 gr were divided into 5 groups (equal). In the control group, no administration was made. The pancreatitis induction groups received 2 doses of 300 and 400 mg/100 g body weight of L-arginine. To the induction groups with two different doses, therapeutic drug was prescribed 3.5 pp. of lipase-pancreatin. All treatments were received for 3 consecutive days once a day from L-arginine solution and 72 hours later, a lipase-pancreatin solution was administered to groups of pancreatitis for 20 days .
Results: The results showed that due to experimental pancreatitis, except for medulla volume, decreasing volume of renal cortex, proximal and distal convoluted tubules, and glomeruli was observed in the treated groups compared to the control group. By the administration of pancreatin lipase along with L-arginine, mean volume values of factors such as mean volume of renal cortex, proximal convoluted tubules and glomeruli, have gotten closed to the control group .
Conclusion: According to a final review of the findings, lipasepancreatin solution may be partially effective in mitigating the effects of pancreatitis induced by high doses of L-arginine 

کلیدواژه‌ها [English]

  • Stereology
  • L-Arginine
  • Pancreatitis
  • Convoluted Tubules
  • Mice
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