مقایسه تأثیر چهار هفته تمرین ورزشی تداومی و تناوبی بر سطوح سرمی و عضلانی آیریزین در موش‌های ماده مبتلا به سرطان پستان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

2 استادیار، گروه تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

زمینه و هدف  آیریزین، یک میوکین مترشحه از عضله اسکلتی است که می‌تواند رشد سلول‌های سرطانی را تحت تأثیر قرار دهد و بر تغییرات توده عضلانی تأثیرگذار باشد. در پژوهش حاضر، تأثیر چهار هفته تمرین ورزشی تداومی و تناوبی بر سطوح سرمی و عضلانی آیریزین در موش‌های ماده مبتلا به سرطان پستان، بررسی شده است.
مواد و روش‌ها تعداد 32 سر موش ماده Balb/c در چهار گروه هشت‌تایی شامل گروه‌های کنترل سالم، کنترل توموری، تمرین تناوبی+تومور و تمرین تداومی+تومور قرار گرفتند. به منظور القای سرطان پستان از رده سلولی MC4-L2 استفاده شد. برنامه تمرین ورزشی (تناوبی و تداومی) به مدت چهار هفته و پنج جلسه در هفته اجرا شد. 48 ساعت بعد از جلسه آخر تمرین، نمونه‌های خونی و بافت عضله دوقلو گرفته شد و به منظور اندازه‌گیری سطوح آیریزین به روش الایزا استفاده شد. تجزیه و تحلیل داده‌ها با آزمون آنالیز واریانس یک‌طرفه و آزمون تعقیبی توکی صورت گرفت و معناداری 0/05≥p در نظر گرفته شد.
یافته‌ها افزایش معنادار سطوح سرمی آیریزین در گروه‌های تمرین تداومی (0/002=p) و تناوبی (0/011=p) در مقایسه با گروه کنترل توموری مشاهده شد. حجم تومور نیز در گروه تداومی و تناوبی در مقایسه با گروه کنترل توموری به صورت معناداری کاهش یافت (0/05≥p)، اما تغییر معناداری در وزن عضله دوقلو بعد از تمرین تداومی و تناوبی مشاهده نشد (0/05<p).
نتیجه‌گیری باتوجه به یافته‌های حاضر می‌توان بخشی از تأثیرات ضدتوموری مشاهده شده به دنبال تمرینات تداومی و تناوبی را با افزایش سطوح آیریزین مرتبط دانست و اینکه تفاوت معناداری بین دو نوع برنامه تمرین ورزشی تداومی و تناوبی وجود ندارد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Comparison the effect of four weeks continuous and interval exercise training on serum and muscular levels of irisin in breast cancer bearing mice

نویسندگان [English]

  • Bentol Hoda asadi 1
  • mandana gholami 2
  • Hossein Abed Natanzi 2
  • nader shakeri 2
1 M.A student, Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Assistant Professor, Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Introduction: Irisin is one of the skeletal muscle secreted myokine that can affect the growth of cancer cells and influence the muscular tissue changes. In the present study, the effect of four weeks continuous and interval exercise training on serum and muscular levels of irisin in breast cancer bearing mice were investigated.
Materials and Methods: The 32 female Balb/c mice assigned in four group with eight mice including: healthy control, tumor control, interval training+tumor and continuous training+tumor groups. In order to induce breast cancer, MC4-L2 cell line were used. Interval and continuous exercise training program conducted in four weeks and five sessions per week. The 48 hours after last exercise training session, blood samples and gastrocnemius muscle tissue were isolated and used for measurement of irisin levels by ELISA method. Data analyze performed by one-way analysis of variance test and Tukey post-hock test and significant considered at p≤0.05.
Results: Significant increase of irisin serum levels in continuous (p=0.002) and interval (p=0.011) training group compared to tumor control group was observed. Tumor volume significantly decreased in continuous and interval training group (p>0.05), but not significant changes in gastrocnemius muscle weight observed after continuous and interval training (p>0.05).
Conclusion: According to present study findings, the observed antitumor effects of continuous and interval training can partly attribute to increase in irisin levels and there is no significant difference between continuous and interval exercise training

کلیدواژه‌ها [English]

  • Breast Cancer
  • Tumor Volume
  • Exercise Training
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