تأثیر هشت هفته تمرین مقاومتی بر ActRIIB میوکارد بطن چپ و سطوح پلاسمایی GDF11 و GDF8 در موش‌های صحرایی نر پیر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دکتری فیزیولوژی ورزشی، دانشکده علوم انسانی، دانشگاه شهرکرد، ایران

2 دانشیار گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه شهرکرد، ایران

3 دانشیار گروه پاتولوژی، دانشگاه علوم پزشکی اصفهان، ایران

چکیده

زمینه و هدف  تمرینات ورزشی با تغییر غلظت فاکتورهای رشدی و گیرنده‌های آن، ممکن است تأثیرات مفیدی بر عضله قلبی داشته باشند و بروز نارسایی قلبی را کاهش دهند. از این رو هدف از این مطالعه، بررسی تأثیر هشت هفته تمرین مقاومتی بر ActRIIB میوکارد بطن چپ و سطوح پلاسمایی GDF11 و GDF8 در موش‌های صحرایی نر پیر بود.
مواد و روش‌ها تعداد 14 سر موش صحرایی نر پیر با محدوده سنی24 تا 27 ماه پس از طی دوره آشناسازی، به صورت تصادفی به دو گروه کنترل (هفت سر) و تمرین (هفت سر) تقسیم شدند. تمرین مقاومتی، شامل هشت هفته و هفته‌ای پنج جلسه صعود از یک نردبان یک متری بود. GDF11 و GDF8 سرم به روش الایزا و ActRIIB بافت بطن چپ به روش IHC اندازه‌گیری شد. داده‌‌های به دست آمده از آزمون  tمستقل، در سطح معناداری کمتر از 05/0 با استفاده از نرم‌افزار SPSS تجزیه و تحلیل شدند.
یافته‌ها مقایسه بین گروهی، اختلاف معناداری، در بین گروه‌‌های تمرین و کنترل در متغیرهای GDF11 (001/0≥p)، GDF8/GDF11 (001/0≥p)، GDF8 (027/0≥p) و وزن قلب (031/0≥p) نشان داد اما اختلاف معناداری در ActRIIβ میوکارد مشاهده نشد (05/0<p).
نتیجه‌گیری به نظر می‌رسد تمرینات مقاومتی با اثرات مطلوب بر عوامل رشدی در هایپرتروفی فیزیولوژیک قلبی سالمندان مؤثر است. از این رو از این تمرینات می‌توان در راستای کاهش بروز نارسایی قلبی بهره برد. با این حال، باید مطالعات بیشتری در این زمینه انجام شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of an Eight-Week Resistance Training on ActRIIβ in the Myocardial Left Ventricular and Plasma Levels of GDF11, GDF8 in Old Male Rats

نویسندگان [English]

  • behnam masoudian 1
  • Akbar Azamian Jazi 2
  • mohammad faramarzi 2
  • ardeshir talebi 3
1 PhD of Exercise Physiology, Faculty of Sport Sciences, Shahrekord University, Iran
2 Department of Sport sciences, Faculty of Sport Sciences, Shahrekord University, Iran
3 Department of Pathology, Isfahan University of Medical Sciences, Iran
چکیده [English]

Introduction: Exercise trainings have beneficial effects on myocardium by changing the concentration of growth factors and their receptors and reduces the risk of heart failure. The aim of this study was to determine the effect of an Eight-week resistance training on ActRIIβin the myocardial left ventricular and plasma levels of GDF11, GDF8 in old male rats.
Materials and Methods: After the familiarization period, fourteen old male rats with age range 24 to 27 months were randomly divided into control (n= Seven) and training (n= Seven) groups. Resistance training was included eight weeks and five sessions per week climbing from a 1-meter ladder. GDF11 and GDF8 were measured by ELISA method and ActRIIβin the myocardial left ventricular was measurement by IHC method. Independent t-test was applied for statistical analysis of the data (p≤0.05).
Results: Comparisons between groups showed a significant difference between the training and control groups in GDF11 (p≤0.001), GDF8/GDF11 (p≤0.001), GDF8 (p≤0.027) and heart weight (p≤0.031). Between groups comparisons not showed a significant difference between the training and control groups in myocardium ActRIIβ (p>0.05).
Conclusion: It seems that resistance training is effective in Seniors cardiac physiological hypertrophy with beneficial effects on growth factors. Therefore, these trainings can be used to reduce the incidence of heart failure; however, more studies are needed in this regard.

کلیدواژه‌ها [English]

  • Resistance training
  • ActRIIβ
  • Old Male Rats
  • GDF11
  • GDF8
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