تأثیر همزمان تمرین هوازی و مکمل‌سازی عصاره گیاهی مرزنجوش بر بیان ژنی پروتئین‌های آپوپتوز میوکارد موش‌های صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی واحد تهران مرکزی، تهران، ایران

2 گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی واحد تهران مرکزی، تهران، ایران

3 دانشیار گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی واحد تهران مرکزی، تهران، ایران

چکیده

چکیده
زمینه: امروزه استفاده از مکمل‌ها و عصاره‌های گیاهی مانند مرزنجوش برای مقابله با آپوپتوز ناشی از ورزش و بهبود سازگارهای‌های تمرینی رواج بسیاری یافته است. بنابراین، مطالعه حاضر با هدف تعیین تأثیر همزمان تمرین هوازی و مکمل‌سازی عصاره گیاهی مرزنجوش بر بیان ژنی پروتئین‌های آپوپتوز میوکارد موش‌های صحرایی انجام شد.
روش‌ کار: در این مطالعه تجربی، 40 سر موش صحرایی نر نژاد ویستار (8 هفته‌ای)‌ با میانگین وزنی (86/17± 129 گرم) به صورت تصادفی در پنج گروه همگن، پایه (8n= )، کنترل(8n= )، عصاره(8n= )، تمرین(8n= ) و تمرین - عصاره (8n= ) قرار گرفتند. گروه تمرین و تمرین-عصاره در یک برنامه 12 هفته‌ای(هر هفته 5 جلسه‌ی 60-10 دقیقه‌ای با شدت80-75 درصد اکسیژن مصرفی بیشینه) دویدن روی نوارگردان حیوانی(شیب 15درصد و سرعت 33-24 متر بر دقیقه) شرکت کردند. همچنین، مکمل‌سازی مرزنجوش شامل دریافت عصاره اتانولی مزرنجوش بود. 48 ساعت پس از آخرین جلسه ‌تمرینی، بخشی از بافت بطن چپ قلب موش‌ها برداشته شد و بیان پروتئین‌هایBax، Bcl-2 و کاسپاز-9 با استفاده از روش RT-PCR بررسی شد. داده‌ها، با استفاده از آزمون تحلیل واریانس دوراهه تجزیه و تحلیل شد(P

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of concurrent aerobic training with Origanum vulgare Hydro Ethanolic Extract supplementation on gene expression of myocardial apoptosis proteins in male rats

نویسندگان [English]

  • Ghader Rahimzadeh 1
  • Mohammadali Azarbaijani 2
  • Hassan Matin homaei 3
1 PhD Student at Islamic Azad University, Central Tehran Branch, Department of Sport Sciences, Faculty of Physical Education and sport sciences, Tehran, Iran
2 Professor at Islamic Azad University, Central Tehran Branch, Department of Sport Sciences, Faculty of Physical Education and sport sciences, Tehran, Iran
3 Associate Professor at Islamic Azad University, Central Tehran Branch, Department of Sport Sciences, Faculty of Physical Education and sport sciences, Tehran, Iran
چکیده [English]

Abstract
Background: Today, the use of supplements and herbal extracts has been widely used to prevent exercise induced apoptosis and improve exercise training adaptations. Thus, the present study examined the effect of the 12 weeks aerobic training with Origanum vulgare ethanolic Extract supplementation on the myocardial apoptosis in young male rats.
Methods: In this experimental study,40 male Wistar rats (8 weeks old)with an average(weight 129±17.86 in gr)randomly divided in five equal groups:basic(n=8),control(n=8),Origanum vulgare(n=8), aerobic training(n=8)and aerobic training-Origanum vulgare(n=8).The aerobic training and aerobic training - Origanum vulgare groups participated in a 12-week program (5 sessions of 10-60 minutes each week, with an intensity of 75-80% of maximum oxygen consumption) running on animal treadmill (15% incline and 24-33 m/s).Also,Origanum vulgare Ethanolic extract was used for supplementation.Forty-eight hours after the last exercise session,a part of the left venticular tissue of the heart was removed and expression of Bax,Bcl2 and caspase-9 proteins was investigated using RT-PCR method. Data were analyzed byTwo-way ANOVA test (P

کلیدواژه‌ها [English]

  • Aerobic Exercise
  • Origanum vulgare
  • apoptosis
  • Rat
Phaneuf S, Leeuwenburgh C. Apoptosis and exercise. Medicine & Science in Sports & Exercise. 2001;33(3):393-6.
Kwak H-B. Effects of aging and exercise training on apoptosis in the heart. Journal of exercise rehabilitation. 2013;9(2):212.
Farrell P, Joyner J, Caiozzo V. ACM’s ADVANCED EXERCISE PHYSIOLOGY. Philadelphia: Lippincott Williams & Wilkins; 2012.
Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell. 1997;91(4):479-89.
Pedersen BK, Steensberg A, Schjerling P. Exercise and interleukin-6. Current opinion in hematology. 2001;8(3):137-41.
Navalta J, Sedlock D, Park K-S. Effect of exercise intensityon exercise-induced lymphocyte apoptosis. International journal of sports medicine. 2007;28(06):539-42.
Kwak H-B, Song W, Lawler JM. Exercise training attenuates age-induced elevation in Bax/Bcl-2 ratio, apoptosis, and remodeling in the rat heart. TheFASEB Journal. 2006;20(6):791-3.
Lee Y, Min K, Talbert EE, Kavazis AN, Smuder AJ, Willis WT, et al. Exercise protects cardiac mitochondria against ischemia-reperfusion injury. Medicine and science in sports and exercise. 2012;44(3):397-405.
PetersonJM, Bryner RW, Sindler A, Frisbee JC, Alway SE. Mitochondrial apoptotic signaling is elevated in cardiac but not skeletal muscle in the obese Zucker rat and is reduced with aerobic exercise. Journal of applied physiology. 2008;105(6):1934-43.
Abdel-Massih RM, Fares R, Bazzi S, El-Chami N, Baydoun E. The apoptotic and anti-proliferative activity of Origanum majorana extracts on human leukemic cell line. Leukemia research. 2010;34(8):1052-6.
Coccimiglio J, Alipour M, Jiang Z-H, Gottardo C, SuntresZ. Antioxidant, antibacterial, and cytotoxic activities of the ethanolic Origanum vulgare extract and its major constituents. Oxidative medicine and cellular longevity. 2016;2016.
Miraj S. Antioxidant, anticancer, antimicrobial potential of Origanum vulgare. Der Pharmacia Lettre. 2016;8(13):89-97.
Vujicic M, Nikolic I, Kontogianni VG, Saksida T, Charisiadis P, Orescanin-Dusic Z, et al. Methanolic extract of Origanum vulgare ameliorates type 1 diabetes through antioxidant, anti-inflammatory and anti-apoptotic activity. British Journal of Nutrition. 2015;113(5):770-82.
Vainshtein A, Kazak L, Hood DA. Effects of endurance training on apoptotic susceptibility in striated muscle. Journal of applied physiology. 2011;110(6):1638-45.
Naito H, Powers SK, Demirel HA, Aoki J. Exercise training increases heat shock protein in skeletal muscles of old rats. Medicine and science in sports and exercise. 2001;33(5):729-34.
Siu PM, Bryner RW, Martyn JK, Alway SE. Apoptotic adaptations from exercise training in skeletal and cardiac muscles. The FASEB Journal. 2004;18(10):1150-2.
Fernandes T, Magalhães FdC, Carmo ECd, Oliveira EMd. Aerobic exercise training inhibits skeletal muscular apoptotic signaling mediated by VEGF-VEGR2 in spontaneously hypertesive rats. Revista Brasileira de Medicina do Esporte. 2012;18(6):412-8.
BayIr H, Kagan VE. Bench-to-bedside review: Mitochondrial injury, oxidative stress and apoptosis–there is nothing more practical than a good theory. Critical care. 2008;12(1):206.
Soberanes S, Panduri V, Mutlu GkM, Ghio A, Bundinger GS, Kamp DW. p53 mediates particulate matter–induced alveolar epithelial cell mitochondria-regulated apoptosis. American journal of respiratory and critical care medicine. 2006;174(11):1229-38.
Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. International journal of food microbiology. 2004;94(3):223-53.
Arunasree K. Anti-proliferative effects of carvacrol on a human metastatic breast cancer cell line, MDA-MB 231. Phytomedicine. 2010;17(8-9):581-8.
Aristatile B, Al-Assaf AH, Pugalendi KV. Carvacrol suppresses the expression of inflammatory marker genes in D-galactosamine-hepatotoxic rats. Asian Pacific journal of tropical medicine. 2013;6(3):205-11.
Yin Q-h, Yan F-x, Zu X-Y, Wu Y-h, Wu X-p, Liao M-c, et al. Anti-proliferative and pro-apoptotic effect of carvacrol on human hepatocellular carcinoma cell line HepG-2. Cytotechnology. 2012;64(1):43-51.
Jamhiri M, Hafizibarjin Z, Ghobadi M, Moradi A, Safari F. Effect of Thymol on Serum Antioxidant Capacity of Rats Following Myocardial Hypertrophy. Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk. 2017;20(4):10-9.
Prasanna R, Ashraf EA, Essam MA. Chamomile and oregano extracts synergistically exhibit antihyperglycemic, antihyperlipidemic, and renal protective effects in alloxan-induced diabetic rats. Canadian journal of physiology and pharmacology. 2016;95(1):84-92.
Khani M, Motamedi P, Dehkhoda MR, Nikukheslat SD, Karimi P. Effect of thyme extract supplementation on lipid peroxidation, antioxidant capacity, PGC-1α content and endurance exercise performance in rats. Journal of the international society of sports nutrition. 2017;14(1):11.
Sellami M, Slimeni O, Pokrywka A, Kuvačić G, Hayes LD, Milic M, et al. Herbal medicine for sports: a review. Journal of the International Society of Sports Nutrition. 2018;15(1):14.
Jafari A, Pourrazi H, Nikookheslat S, Baradaran B. Effect of exercise training on Bcl-2 and bax gene expression in the rat heart. Gene, Cell and Tissue. 2015;2(4).
Song W, Kwak H-B, Lawler JM. Exercise training attenuates age-induced changes in apoptotic signaling in rat skeletal muscle. Antioxidants & redox signaling. 2006;8(3-4):517-28.
Kandeil MA, Hassanin KM, Mohammed ET, Safwat GM, Mohamed DS. Wheat germ and vitamin E decrease BAX/BCL-2 ratio in rat kidney treated with gentamicin. Beni-Suef University Journal of Basic and Applied Sciences. 2018.
Zhu L, Han MB, Gao Y, Wang H, Dai L, Wen Y, et al. Curcumin triggers apoptosis via upregulation of Bax/Bcl-2 ratio and caspase activation in SW872 human adipocytes. Molecular medicine reports. 2015;12(1):1151-6.