نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموختة گروه آموزشی زیست‌شناسی، واحد شیراز، دانشگاه آزاد اسلامی ، شیراز، ایران

2 دانشیار، گروه آموزشی زیست‌شناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران

چکیده

اهداف: حافظه و یادگیری یکی از اعمال پیچیده مغزی و مکانیسمی برای کدبندی، ذخیره سازی و فراخوانی دوباره اطلاعات یادگرفته شده می باشد.با توجه شیوع اختلالات یادگیری به ویژه در سنین بالا این مطالعه با هدف بررسی اثر عصاره هیدروالکلی بابونه بر میزان حافظه اجتنابی موشهای صحرایی انجام گردید.
مواد و روش ها: در این مطالعه تجربی از 48 موش صحرایی نر بالغ که به 6 گروه ‌شامل کنترل، شاهد1(تیمار با سالین)، شاهد 2 (قرار گرفتن در شاتل باکس) و سه دسته تجربی دریافت کننده درون صفاقی دوزهای mg/kg100،200 و400 عصاره هیدروالکلی گیاه بابونه تقسیم شدند استفاده گردید. تجویزها برای 15روز انجام گردید و جهت سنجش میزان حافظه و یادگیری از دستگاه شاتل باکس استفاده شد و مدت زمان تاخیر در ورود و مدت زمان ماندن در اتاقک تاریک شاتل باکس ثبت گردید. نتایج با استفاده از نرم افزار22- SPSS و با کمک آزمون‌های ANOVA و توکی آنالیز گردیدند. معناداری اختلاف دادهها در سطح05/0≥p در نظر گرفته شد.
نتایج: نتایج حاصل از آنالیز دادهها نشان داد دوزهای
mg/kg200 و400 عصاره بابونه باعث افزایش معنادار در مدت زمان تاخیر در ورود به بخش تاریک و دوزهای mg/kg100، 200و400 عصاره بابونه باعث کاهش معنادار در مدت زمان حضور در بخش تاریک شاتل باکس در مرحله به خاطرآوری در سطح 001/0P≤ نسبت به گروه کنترل می گردد
نتیجه گیری : نتایج نشان داد عصاره بابونه باعث تقویت حافظه اجتنابی در موشهای صحرایی می شود که این اثر را احتمالا به دلیل داشتن ترکیبات فلاونوئیدی، آنتی اکسیدانی و فیتواستروژنیک اعمال می نماید.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of hydroalcoholic Chamomile (Atricaria chamomilla) extract on avoidance memory in Adult Male Rats

نویسندگان [English]

  • Fatemea Seyf 1
  • Seyed Ebrahim Hosseini 2

1 M.Sc. Department of Biology, Sciences Faculty, Shiraz Branch, Islamic Azad University, Shiraz, Iran

2 Associate Professor, Department of Biology, Sciences Faculty, Shiraz Branch, Islamic Azad University, Shiraz, Iran

چکیده [English]

Background:Memory and learning are one of the most complex brain functions and a mechanism for encoding, storing and recalling learned information. Due to the prevalence of learning disabilities, especially in older adults, the present study aimed to investigate the effect of hydroalcoholic extract of chamomile on avoidance memory levels in rats.
Materials and Methods:In this experimental study, 48 adult male rats were divided into 6 groups: control, control 1 (treatment with normal saline) and control 2 (Shuttle Box) and three experimental groups receiving 200,100, and 400 mg/kg hydroalcoholic extract of chamomile by intraperitoneal injections. All of them were prescribed for 15 days. In this study, the Shuttle Box was used to measure memory and learning. The latency of entering and the time of remaining in a dark chamber of the Shuttle Box were recorded. The results were analyzed by ANOVA and Tukey tests using the SPSS-22 software.
Results:The significance level of data was considered as p≤0.05. The results showed that doses of 200 and 400 mg/kg chamomile extract significantly increased the latency of entering the dark chamber and also doses of 100, 200, and 400 mg/kg chamomile extract significantly reduced the time of remaining in the dark chamber of the Shuttle Box in the remembering phase at the level of P≤0.001 compared to the control group.
Conclusions: The results of this study showed that chamomile extract strengthens avoidance memory in rats. This effect is probably due to flavonoids, antioxidants and phytoestrogens.

کلیدواژه‌ها [English]

  • Chamomile
  • Memory
  • Rat
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