تأثیر تمرین استقامتی بر پروتئین–D سورفکتانت (SPD) و شاخص مقاومت به انسولین موش‎های صحرایی نر دیابتی نوع 2

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه سیستان و بلوچستان، زاهدان، ایران.

2 استادیار، گروه فیزیولوژی و آسیب شناسی ورزشی، دانشکده علوم ورزشی، دانشگاه اراک، اراک، ایران

چکیده

اهداف: ﻫﺪف از ﭘﮋوﻫﺶ ﺣﺎﺿﺮ، ﺑﺮرﺳﯽ اثر 8هفته تمرین استقامتی بر سطوح سرمی پروتئین–D سورفکتانت (SPD) موش‎های صحرایی پس از القاء دیابت توسط استرپتوزوتوسین- نیکوتین آمید بود.
مواد و روش‏ها: در این مطالعه تجربی 48 ﺳﺮ موش صحرایی از ﻧﮋاد وﯾﺴﺘﺎر ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ وزن 48±200 ﮔﺮم ﺑﻪﻃﻮر ﺗﺼﺎدﻓﯽ در چهار ﮔﺮوه ﮐﻨﺘﺮل سالم، کنترل دیابتی، سالم تمرین استقامتی و دیابتی ﺗﻤﺮﯾﻦ استقامتی ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﺟﻬﺖ اﻟﻘﺎی دﯾﺎﺑﺖ، از روش ﺗﺰرﯾﻖ درون ﺻﻔﺎﻗﯽ mg/kg 120 ﻣﺤﻠﻮل نیکوتین آمید به همراه mg/kg 65 ﻣﺤﻠﻮل STZ اﺳﺘﻔﺎده ﮔﺮدﯾﺪ. ﭘﺮوﺗﮑﻞ ﺗﻤﺮﯾﻦ اﺳﺘﻘﺎﻣﺘﯽ ﺑﺎ ﺷﺪت ﻣﺘﻮﺳﻂ ﺑﻪ ﻣﺪت 8 ﻫﻔﺘﻪ اﻧﺠﺎم ﮔردید و 24 ﺳﺎﻋﺖ ﭘﺲ از آﺧﺮﯾﻦ ﺟﻠﺴﮥ ﺗﻤﺮﯾﻨﯽ ﺣﯿﻮاﻧﺎت ﺗﺸﺮﯾﺢ و سرم خون موش‎های صحرایی جهت بررسی جمع آوری شدند. داده‎ﻫﺎ ﺑﺎ اﺳﺘﻔﺎده از آزﻣﻮن ﺗﺤﻠﯿﻞ وارﯾﺎﻧﺲ ﯾﮏ ﻃﺮﻓﻪ (one-way ANOVA) و آزمون تعقیبی توکی در سطح معناداری 05/0 بررسی شدند.
یافته‏ها: تمرین استقامتی موجب کاهش معنا‎دار قند خون گروه تمرین استقامتی دیابتی نسبت به گروه کنترل دیابتی شد (000/0=P). سطوح SPD در گروه کنترل دیابتی نسبت به کنترل سالم کاهش معناداری (001/0=P) و شاخص مقاومت به انسولین (HOMA-IR) افزایش معناداری (000/0=P) یافت. 8 هفته تمرین استقامتی موجب افزایش معنادار درSPD (041/0P=) در گروه تمرین استقامتی دیابتی نسبت به گروه کننترل دیابتی شد. همچنین تمرین موجب کاهش معنادار شاخص مقاومت به انسولین (000/0=P) و انسولین (029/0=P) در گروه تمرین استقامتی دیابتی نسبت به گروه کنترل دیابتی شد.
نتیجه‌گیری: به نظر می‎رسد تمرین استقامتی از طریق افزایش سطوح سرمSPD موجب بهبود شاخص مقاومت به انسولین در موش‎های دیابتی نوع 2 می‎شود.
کلید واژه‏ها: استرپتوزوتوسین- نیکوتین‏آمید، پروتئین– D سورفکتانت، انسولین، تمرین.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of Endurance Training on Protein-D Surfactant (SPD) and Insulin Resistance Index in Type 2 Diabetic Rats

نویسندگان [English]

  • Abbas Salhikia 1
  • Mohammad Parastesh 2
1 Assistant Professor, Department of Sport Sciences, Faculty of Education and Psychology, University of Sistan and Baluchestan, Zahedan, Iran.
2 Assistant professor, Sport physiology and Injury group, Faculty of Sport Sciences, Arak University, Arak, Iran.
چکیده [English]

Background: The aim of present study was to investigate effects of endurance training on surfactant protein-D in streptozotocin–nicotinamide induced diabetic rats.
Materials and Methods: In this experimental study، thirty-six adult male Wistar rats in the weight range of 200±48gr, randomly assigned in to four groups: healthy control, diabetic control, diabetic endurance training and healthy endurance training. For induction of diabetes, intraperitoneal injection of nicotinamide solution (120 mg/kg) and STZ solution (65 mg/kg) was used. The endurance training protocol was performed for 8-week and Twenty-four hours after the last training session blood serum samples were examined for evaluating reproductive hormones. Data were analyzed using one-way ANOVA and Turkey’s Post Hoc test at 0.05%.
Results: Endurance training caused a significant decrease in blood glucose in the endurance diabetic training group compared to the diabetic control group (P=0.000). Serum Protein-D Surfactant (SPD) serum levels in the diabetic control group significantly decreased (P=0.001) and the insulin resistance index (P=0.000) was significantly lower than healthy controls. 8 weeks of endurance training significantly increased in SPD (P=0.041) in endurance diabetic training group compared to diabetic control group. Also, exercise significantly decreased insulin resistance index (P=0.000) and insulin (P=0.029) in endurance diabetic training group compared to diabetic control group.

Conclusion: It seems that Endurance training by increasing the levels of serum SPD improve insulin resistance index in type 2 diabetic mice.
Keywords: Streptozotocin–nicotinamide, Surfactant protein-D, Insulin, Training.

کلیدواژه‌ها [English]

  • Keywords: Streptozotocin–nicotinamide
  • Surfactant protein-D
  • Insulin
  • training
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