تأثیر دو برنامه تمرین مقاومتی و استقامتی فزاینده بر بیان ژن FNDC5 عضله دوقلو در رت های نر

حسین‌زاده, محمد; رشیدلمیر, امیر; حجازی, سید محمود

نوع مقاله : مقاله پژوهشی

نویسندگان

¹ گروه تربیت بدنی، دانشگاه فرهنگیان، تهران، ایران

² دانشیار گروه فیزیولوژی ورزشی دانشکده تربیت بدنی و علوم ورزشی دانشگاه فردوسی مشهد، مشهد، ایران

³ گروه تربیت بدنی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران

چکیده

زمینه و هدف: پروتئین تراغشایی فیبرونکتین نوع 3 دامنه حاوی پروتئین 5 (FNDC5) به‌عنوان یک فاکتور مهم برای تمایز سلولی عضله اسکلتی شناخته شده است. باتوجه به اینکه اثرات مثبت ورزش منظم از طریق عضله اسکلتی منتقل می‌شود، هدف ازپژوهش حاضر بررسی اثر دو برنامه تمرین مقاومتی واستقامتی فزاینده بر بیان ژن FNDC5 عضله دوقلو در رت‌های نر بود.
مواد و روش‌ها: در این پژوهش 15سر موش صحرایی نر به‌طور تصادفی به سه‌گروه کنترل (5 سر)، تمرین استقامتی (5 سر) و تمرین مقاومتی (5 سر)، تقسیم شدند.گروه استقامتی تمرینات را به مدت 8 هفته روی نوارگردان وگروه مقاومتی نیز تمرینات مقاومتی را به مدت هشت هفته روی نردبان یک متری با شیب 85 درجه انجام دادند.72 ساعت پس از پایان تمرین حیوانات بیهوش شده، بافت عضله دوقلو جدا و اندازه‌گیری بیان ژن FNDC5 انجام شد. ازآزمون آنالیز واریانس یک‌طرفه برای تجزیه وتحلیل داده‌‌ها استفاده و معنی‌داری درسطح 05/0 ≥P پذیرفته شد.
یافته‌ها:اختلاف معنی‌داری در بیان ژن FNDC5 در بین گروه‌ها مشاهده شد)001/0 P= و 791/31 (F=. به‌کارگیری آزمون تعقیبی Tukey نشان داد، بیان ژن FNDC5 عضله دوقلو درگروه تمرین استقامتی و تمرین مقاومتی نسبت به گروه کنترل به‌طور معنی‌داری (به ترتیب 001/0P

کلیدواژه‌ها

موضوعات

فیزیولوژی ورزشی

عنوان مقاله [English]

The Effect of Progressive Resistance and Endurance Training on Gastrocnemius Muscle’s FNDC5 Gene Expression in Male Rats

نویسندگان [English]

Mohammad Hoseinzadeh ¹
Amir Rashidlamir ²
Seyed Mahmud Hejazi ³

¹ Department of physical education, Farhangian university, Tehran, Iran

² Department of physical education, Ferdowsi University of Mashhad, Mashhad, Iran

³ Department of physical education, Mashhad branch, Islamic Azad University, Mashhad, Iran

چکیده [English]

Background and purpose: Fibronectin transmembrane type III domain-containing protein 5 (FNDC5) is a characteristic factor in distinguishing skeletal muscle cells. Since the productive effects of regular exercise are conveyed through the skeletal muscle, this study aims to compare the effects of progressive endurance and resistance training on FNDC5 gene expression in male rats’ gastrocnemius muscles.
Materials and Methods: 15 male rats were randomly assigned to three equal groups of: control, endurance training and resistance training. The endurance group performed 8 weeks of training on the treadmill. The resistance group did their exercises for eight weeks on a three-session-per-week basis on a one-meter-high ladder with 85○ slope. All subjects were anesthetized 72 hours after the experiment finished and the gastrocnemius removed. The level of FNDC5 expression was measured through real-time PCR method and one-way ANOVA applied to analyze the data. Level of significance was set to P≥0.05.
Results: One-way ANOVA showed a significant difference in FNDC5 expression among the groups (P=0.001, F=31.791). A subsequent Tukey test application showed FNDC5 gene expression of the gastrocnemius muscle was significantly higher in the resistance and endurance training groups than in the control group (P

کلیدواژه‌ها [English]

FNDC5
Endurance Training
Resistance training

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مجله دانشگاه علوم پزشکی سبزوار

تأثیر دو برنامه تمرین مقاومتی و استقامتی فزاینده بر بیان ژن FNDC5 عضله دوقلو در رت های نر

مراجع

مراجع

دوره 25، شماره 5
آذر و دی 1397
صفحه 629-637

تأثیر دو برنامه تمرین مقاومتی و استقامتی فزاینده بر بیان ژن FNDC5 عضله دوقلو در رت های نر

مراجع

مراجع

دوره 25، شماره 5آذر و دی 1397صفحه 629-637

دوره 25، شماره 5
آذر و دی 1397
صفحه 629-637