نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه تربیت بدنی ، واحد کرمان ، دانشگاه آزاد اسلامی ، کرمان ،ایران

2 دانشیار گروه فیزیولوژی ورزشی دانشکده تربیت بدنی و علوم ورزشی دانشگاه فردوسی مشهد، مشهد، ایران

3 استادیار پژوهشگاه تربیت بدنی و علوم ورزشی

چکیده

زمینه و هدف: برنامه بازتوانی قلبی در راستای کاهش خطرات ثانویه و افزایش عملکرد بیماران تحت عمل بای پس قلبی طراحی شده که نهایتا با تاخیر و کاهش میزان مرگ و میر قلبی همراه است. یک بخش کلیدی از این برنامه فعالیت ورزشی است. هدف از مطالعه حاضر، بررسی تغییرات بیان ژن ABCG8 سلول‌های PBMN در زنان میانسال پس از بای‌پس عروق کرونری متعاقب هشت هفته تمرین مقاومتی در آب و خشکی است.در این پژوهش 30 زن میانسال بیمار قلبی-عروقی به سه گروه کنترل، تمرین مقاومتی در آب و در خشکی تقسیم شدند. برنامه تمرین مقاومتی به مدت هشت هفته (3 جلسه در هفته) با شدت 60 درصد یک تکرار بیشینه اجرا گردید. گروه کنترل در این مدت در هیچ برنامه تمرینی شرکت نداشتند. 48ساعت قبل از شروع برنامه تمرینی و 48ساعت بعد از آخرین جلسه تمرینی از آزمودنی‌ها درحالت ناشتا خونگیری شد و جداسازی سلول‌های PBMN جهت اندازه‌گیری‌ بیان ژن ABCG8 انجام گردید. برای تعیین تفاوت‌های درون‌گروهی و بین‌گروهی، از آزمون آنالیز واریانس با اندازه‌های تکراری و آزمون تی‌همبسته استفاده شد و سطح معنی‌داری 05/0P≤ درنظر گرفته شد. نتایج آزمون آنالیز واریانس با اندازه‌های تکراری نشان داد، اختلاف بین‌گروهی معنی‌داری در بیان نسبی ژن ABCG8 وجود دارد (001/0 P=). همچنین نتایج آزمون تی‌همبسته نشان داد اختلاف درون‌گروهی معنی‌داری در بیان ژن ABCG8 در گروه‌ تمرین مقاومتی در آب و تمرین مقاومتی در خشکی وجود دارد(بترتیب؛001/0 P= و 001/0 P=). نتایج پژوهش حاضر نشان می‌دهد هر دو نوع مدل تمرینی، سازگاری‌های مطلوب مشابهی را بر روی بیان ژن ABCG8 القاء می‌کند و لذا از این طریق ممکن است به طور غیر مستقیم مانع تجمع کلسترول در عروق کرونر شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Changes of ATP –binding cassette transporter type G8 gene expression subsequent eight weeks of quatic and dryland resistance training in middle-aged women’s Peripheral blood cells after coronary artery bypass grafting

نویسندگان [English]

  • Leili Zeiaadini Dashtkhaki 1
  • Amir Rashidlamir 2
  • Said Naghibi 3

1 Department of physical education,Kerman branch, Islamic Azad University, Kerman, Iran

2 Department of physical education, Ferdowsi University of Mashhad, Mashhad, Iran

3 Physical Education and Sport Science Research Center, Tehran, Iran

چکیده [English]

Cardiac rehabilitation program aimed at reducing secondary risk factors and improving function in patients undergoing coronary artery bypass grafting run which finally may delay or reduce mortality patients. A key component of this program is exercise. The aim of this study was to evaluate the effect of aquatic and dryland training on ABCG8 gene expression in middle-aged women’s peripheral blood mononuclear cells after coronary artery bypass grafting. 30 middle-aged women with cardiovascular disease were divided into three groups including control, aquatic and dryland resistance training. The program lasted for eight weeks of resistance training (3 sessions per week/ 60 minutes per session) and started with 60% of 1RM. Fasting blood samples were taken from all participants 48 hours both prior to the beginning of the training program and after the last training session. ABCG8 relative gene expression was detected by Real-time PCR method. Repeated measures and paired t-test with a significance level of p≤ 0.05 were used to determine differences between and within groups. Repeated measures test showed a significant increase in the relative ABCG8 gene expression in both the aquatic and dryland training groups (P= 0.001). Moreover, paired-samples t-test showed a significant difference in aquatic and dryland training groups regarding ABCG8 gene expression (P= 0.001; P= 0.001). It can be concluded that both training programs on land and water training, increase ABCG8 gene expression in middle-aged women after coronary artery bypass and a possible reverse cholesterol transport process can be improved.

کلیدواژه‌ها [English]

  • ATP–binding cassette transporter typeG8
  • Resistance training
  • Coronary artery bypass grafting
  • Female
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